Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

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dc.contributor.authorLuong, John H. T.
dc.contributor.authorVashist, Sandeep Kumar
dc.date.accessioned2018-01-17T11:09:53Z
dc.date.available2018-01-17T11:09:53Z
dc.date.issued2015-11-18
dc.description.abstractTwo-dimensional (2D) graphene, sp2-hybridized carbon, and its two major derivatives, graphene oxide (GO) and reduced graphene oxide (rGO) have played an important role in immunoassays (IAs) and immunosensing (IMS) platforms for the detection of carcinoembryonic antigen (CEA), an implicated tumor biomarker found in several types of cancer. The graphene family with high surface area is functionalized to form stable nanocomposites with gold nanoparticles (AuNPs) and electron mediators. The capture anti-CEA antibody (Ab) with high density can be anchored on AuNPs of such composites to provide remarkable detection sensitivity, significantly below the level found in normal subjects and cancer patients. Electrochemical and fluorescence/chemiluminescence-quenching properties of graphene-based nanocomposites are exploited in various detection schemes. Future endeavors are envisioned for the development of an array platform with high-throughput for CEA together with other tumor biomarkers and C-reactive protein, a universal biomarker for infection and inflammation. The ongoing efforts dedicated to the replacement of a lab-based detector by a cellphone with smart applications will further enable cost-effective and frequent monitoring of CEA in order to establish its clinical relevance and provide tools for real-time monitoring of patients during chemotherapy.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLuong, J. H. T. and Vashist, S. K. (2017) ‘Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites’, Biosensors and Bioelectronics, 89(1), pp. 293-304. doi: 10.1016/j.bios.2015.11.053en
dc.identifier.doi10.1016/j.bios.2015.11.053
dc.identifier.endpage304en
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.issued1en
dc.identifier.journaltitleBiosensors and Bioelectronicsen
dc.identifier.startpage293en
dc.identifier.urihttps://hdl.handle.net/10468/5285
dc.identifier.volume89en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.rights© 2017, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectGrapheneen
dc.subjectGraphene oxideen
dc.subjectReduced graphene oxideen
dc.subjectCarcinoembryonic antigenen
dc.subjectImmunoassayen
dc.subjectImmunosensing platformen
dc.subjectElectrochemical sensingen
dc.subjectChemiluminescenceen
dc.subjectFluorescence quenchingen
dc.titleImmunosensing procedures for carcinoembryonic antigen using graphene and nanocompositesen
dc.typeArticle (peer-reviewed)en
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