A systematic analysis of the role of GGDEF-EAL domain proteins in virulence and motility in Xanthomonas oryzae pv. oryzicola

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dc.contributor.authorWei, Chao
dc.contributor.authorJiang, Wendi
dc.contributor.authorZhao, Mengran
dc.contributor.authorLing, Junjie
dc.contributor.authorZeng, Xin
dc.contributor.authorDeng, Jun
dc.contributor.authorJin, Dongli
dc.contributor.authorDow, J. Maxwell
dc.contributor.authorSun, Wenxian
dc.contributor.funderSpecial Fund for Agro-scientific Research in the Public Interest
dc.contributor.funderNational Natural Science Foundation of China
dc.contributor.funderNational High Technology Research and Development Program of China
dc.contributor.funder111 project
dc.date.accessioned2016-10-11T08:47:08Z
dc.date.available2016-10-11T08:47:08Z
dc.date.issued2016-04-07
dc.description.abstractThe second messenger c-di-GMP is implicated in regulation of various aspects of the lifestyles and virulence of Gram-negative bacteria. Cyclic di-GMP is formed by diguanylate cyclases with a GGDEF domain and degraded by phosphodiesterases with either an EAL or HD-GYP domain. Proteins with tandem GGDEF-EAL domains occur in many bacteria, where they may be involved in c-di-GMP turnover or act as enzymatically-inactive c-di-GMP effectors. Here, we report a systematic study of the regulatory action of the eleven GGDEF-EAL proteins in Xanthomonas oryzae pv. oryzicola, an important rice pathogen causing bacterial leaf streak. Mutational analysis revealed that XOC_2335 and XOC_2393 positively regulate bacterial swimming motility, while XOC_2102, XOC_2393 and XOC_4190 negatively control sliding motility. The ΔXOC_2335/XOC_2393 mutant that had a higher intracellular c-di-GMP level than the wild type and the ΔXOC_4190 mutant exhibited reduced virulence to rice after pressure inoculation. In vitro purified XOC_4190 and XOC_2102 have little or no diguanylate cyclase or phosphodiesterase activity, which is consistent with unaltered c-di-GMP concentration in ΔXOC_4190. Nevertheless, both proteins can bind to c-di-GMP with high affinity, indicating a potential role as c-di-GMP effectors. Overall our findings advance understanding of c-di-GMP signaling and its links to virulence in an important rice pathogen.en
dc.description.sponsorshipSpecial Fund for Agro-Scientific Research in the Public Interest of China (Grant 201303015); National Natural Science Foundation of China (Grant 31272007); National High Technology Research and Development Program of China (Grant 2012AA100703); 111 project (Grant B13006)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid23769
dc.identifier.citationWei, C., Jiang, W., Zhao, M., Ling, J., Zeng, X., Deng, J., Jin, D., Dow, J. M. and Sun, W. (2016) 'A systematic analysis of the role of GGDEF-EAL domain proteins in virulence and motility in Xanthomonas oryzae pv. oryzicola', Scientific Reports, 6, 23769 (14 pp). doi:10.1038/srep23769en
dc.identifier.doi10.1038/srep23769
dc.identifier.endpage14en
dc.identifier.issn2045-2322
dc.identifier.issued6en
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/3173
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectVirulenceen
dc.subjectProteinsen
dc.subjectMotilityen
dc.subjectBacteriaen
dc.subjectPathogenen
dc.titleA systematic analysis of the role of GGDEF-EAL domain proteins in virulence and motility in Xanthomonas oryzae pv. oryzicolaen
dc.typeArticle (peer-reviewed)en
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