Analysis of expression quantitative trait loci in D. melanogaster
| dc.check.embargoformat | Both hard copy thesis and e-thesis | en |
| dc.check.entireThesis | Entire Thesis Restricted | |
| dc.check.opt-out | No | en |
| dc.check.reason | This thesis is due for publication or the author is actively seeking to publish this material | en |
| dc.contributor.advisor | Moore, Thomas F. | en |
| dc.contributor.advisor | Furlong, Eileen | en |
| dc.contributor.author | Harnett, Dermot | |
| dc.date.accessioned | 2016-09-23T10:06:46Z | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016 | |
| dc.description.abstract | Expression Quantitative Trait Loci (eQTL) analysis allows for the identification of genetic variation associated with variation in gene expression. It is often unclear however, which of the associated variants are causal, and by what mechanism. Integrating functional genomic data with eQTL data can provide insight into the impact of natural variation in the population, and the nature of the transcriptional machinery itself. In this thesis, I integrate functional genomic data with eQTL data derived from both 5’ CAGE and 3’ TagXseq expression assays, in developing embryos. I first use both datasets to analyse the transcription landscape in embryonic D., melanogaster, and then carry out an analysis of sequence motifs associated with transcription factor binding sites, promoters, and 3’ polyadenylation sites. Finally, I integrate functional genomic data, including these novel sequence motifs, to shed light on the mechanisms of gene expression variation in D.,melanogaster. I am able to demonstrate that some variants effecting gene regulation in Drosophila are found within haplotypes which buffer their effects. | en |
| dc.description.status | Not peer reviewed | en |
| dc.description.version | Accepted Version | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Harnett, D. 2016. Analysis of expression quantitative trait loci in D. melanogaster. PhD Thesis, University College Cork. | en |
| dc.identifier.endpage | 204 | en |
| dc.identifier.uri | https://hdl.handle.net/10468/3114 | |
| dc.language.iso | en | en |
| dc.publisher | University College Cork | en |
| dc.rights | © 2016, Dermot Harnett. | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | en |
| dc.subject | Genetics | en |
| dc.subject | Drosophila | en |
| dc.thesis.opt-out | false | |
| dc.title | Analysis of expression quantitative trait loci in D. melanogaster | en |
| dc.type | Doctoral thesis | en |
| dc.type.qualificationlevel | Doctoral | en |
| dc.type.qualificationname | PhD (Science) | en |
| ucc.workflow.supervisor | t.moore@ucc.ie |
