Exploiting the diverse microbial ecology of marine sponges

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dc.check.embargoformatBoth hard copy thesis and e-thesisen
dc.check.entireThesisEntire Thesis Restricted
dc.check.opt-outNot applicableen
dc.check.reasonThis thesis is due for publication or the author is actively seeking to publish this materialen
dc.contributor.advisorDobson, Alan D. W.en
dc.contributor.advisorMorrissey, John P.en
dc.contributor.authorJackson, Stephen A.
dc.contributor.funderMarine Instituteen
dc.date.accessioned2013-04-09T11:23:12Z
dc.date.available2016-04-09T04:00:05Z
dc.date.issued2013
dc.date.submitted2013
dc.description.abstractMarine sponges (phylum Porifera) are the oldest extant metazoan animals on earth and host large populations of symbiotic microbes: Bacteria, Archaea and unicellular Eukaryota. Those microbes play ecological functions which are essential to the health of the host including carbon, nitrogen and sulfur cycling as well as host defence through the production of bioactive secondary metabolites which protect against infection and predation. The diversity of sponge-associated microbes is remarkable with thousands of OTUs reported from individual sponge species. Amongst those populations are sponge-specific microbes which may be specific to sponges or specific to sponge species. While marine natural product discovery concerns many animal phyla, Porifera account for the largest proportion of novel compounds. Evidence suggests that many of these compounds are the products of symbiotic microbes. Descriptions of sponge-associated microbial community structures have been advanced by the development of next-generation sequencing technologies while the discovery and exploitation of sponge derived bioactive compounds has increased due to developments in sequence-based and function-based metagenomics. Here, we use pyrosequencing to describe the bacterial communities associated with two shallow, temperate water sponges (Raspailia ramosa and Stelligera stuposa) from Irish coastal waters and to describe the bacterial and archaeal communities of a single sponge species (Inflatella pellicula) from two different depths in deep waters in the Atlantic Ocean, including at a depth of 2900m, a depth far greater than that of any previous sequence-based sponge-microbe investigation. We identified diverse microbial communities in all sponges and the presence of sponge-specific taxa recruiting to previously described and novel spongespecific clusters. We also identified archaeal communities which dominated sponge-microbe communities. We demonstrate that sponge-associated microbial communities differ from seawater communities indicating host selection processes. We used sequence-based metagenomic techniques to identify genes of potential industrial and pharmacological interest in the metagenomes of various sponge species and functionbased metagenomic screening in an attempt to identify lipolytic and antibacterial activities from metagenomic clones from the metagenome of the marine sponge Stelletta normani. In addition we have cultured diverse bacterial species from sponge tissues, many of which display antimicrobial activities against clinically relevant bacterial and yeast test strains. Other isolates represent novel species in the genus Maribacter and require emendments to the description of that genus.en
dc.description.sponsorshipMarine Institute (Beaufort Marine Research Award)en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJackson, S. A. 2013. Exploiting the diverse microbial ecology of marine sponges. PhD Thesis, University College Cork.en
dc.identifier.endpage290
dc.identifier.urihttps://hdl.handle.net/10468/1037
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2013, Stephen A. Jacksonen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectMarine spongesen
dc.subjectPoriferaen
dc.subjectMetazoaen
dc.subjectSymbiotic microbesen
dc.subjectSponge derived bioactive compoundsen
dc.subjectMetagenomicsen
dc.subjectPyrosequencingen
dc.subjectRaspailia ramosaen
dc.subjectStelligera stuposaen
dc.subjectInflatella pelliculaen
dc.subjectStelletta normanien
dc.subjectMaribacteren
dc.subject.lcshSpongesen
dc.thesis.opt-outfalse*
dc.titleExploiting the diverse microbial ecology of marine spongesen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Science)en
ucc.workflow.supervisora.dobson@ucc.ie*
ucc.workflow.supervisorcora@ucc.ie*
ucc.workflow.supervisorcora@ucc.ie*
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