The restorative effect of apocynin and catalase in l-arginine induced hypotension on normotensive subjects the role of oxidative stress

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dc.contributor.authorAbdulla, Mohammed
dc.contributor.funderUniversiti Sains Malaysia, 1001/PFARMASI/811347
dc.date.accessioned2026-01-24T12:08:35Z
dc.date.available2026-01-24T12:08:35Z
dc.date.issued2020
dc.description.abstractL-arginine is a substrate for nitric oxide synthase (NOS) responsible for the production of NO. This investigation studied the effect of pocynin, an NADPH oxidase inhibitor and catalase, an H2O2 scavenger on L-arginine-induced oxidative stress and hypotension. Forty Wistar-Kyoto rats were treated for 14 days with vehicle, L-arginine (12.5 mg/ml p.o.), L-arginine + apocynin (2.5 mmol/l p.o.), L-arginine + catalase (10000 U/kg/day i.p.) and L-arginine plus apocynin + catalase respectively. Weekly renal functional and hemodynamic parameters were measured and kidneys harvested at the end of the study for istopathological and renal NADPH oxidase 4 (Nox4) assessments. L-arginine administration in normotensive rats decreased systolic blood pressure (120±2 vs. 91±2 mmHg) and heart rate (298±21 vs. 254±15 bpm), enhanced urinary output (21.5±4.2 vs. 32±1.9 ml/24 h, increased creatinine clearance (1.72±0.56 vs. .62±0.40 ml/min/kg), and fractional sodium excretion (0.88±0.16 vs. 1.18±0.16 %), caused proteinuria (28.10±1.93 vs. 35.26±1.69 mg/kg/day) and a significant decrease in renal cortical blood perfusion (292±3 vs. 258±5 bpu) and pulse wave velocity (3.72±0.20 vs 2.84±0.13 m/s) (all P<0.05). L-arginine increased plasma alondialdehyde (by ~206 % P<0.05) and NO (by ~51 %, P<0.05) but decreased superoxide dismutase (by ~31 %, P<0.05) and total antioxidant capacity (by ~35 %, P<0.05) compared to control. Renal Nox4 mRNA activity was approximately 2.1 fold higher (P<0.05) in the L-arginine-treated rats but was normalized by apocynin and apocynin plus catalase treatment. Administration of apocynin and catalase, but not catalase alone to rats fed L-arginine, restored the deranged renal function and structure, prevented hypotension and enhanced the antioxidant capacity and suppressed Nox4 expression. These findings suggest that apocynin and catalase might be used prophylactically in the states of oxidative stress.en
dc.description.sponsorshipResearch Universiti grant from Universiti Sains Malaysia number 1001/PFARMASI/811347
dc.description.versionPublished Version
dc.format.mimetypeapplication/pdfen
dc.identifier.authororcidAbdulla, Mohammed§0000-0001-5496-5017
dc.identifier.citationAbdulla, M 2020, 'The restorative effect of apocynin and catalase in l-arginine induced hypotension on normotensive subjects the role of oxidative stress', Physiological Research, vol. 69, pp. 1051-1066. https://doi.org/10.33549//physiolres.934426
dc.identifier.doi10.33549//physiolres.934426
dc.identifier.endpage1066
dc.identifier.issn0862-8408
dc.identifier.journaltitlePhysiological Research
dc.identifier.otherORCID: /0000-0001-5496-5017/work/198889866
dc.identifier.startpage1051
dc.identifier.urihttps://hdl.handle.net/10468/18456
dc.identifier.volume69
dc.language.isoen
dc.publisherCzech Academy of Sciences
dc.relation.urihttps://openalex.org/W3104205951
dc.rightsCopyright 2020, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
dc.rights.accessrightsopen access
dc.rights.licensenameAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.statusPeer reviewed
dc.subjectApocynin
dc.subjectMalondialdehyde
dc.subjectEndocrinology
dc.subjectInternal medicine
dc.subjectChemistry
dc.subjectArginine
dc.subjectOxidative stress
dc.subjectCatalase
dc.subjectSuperoxide dismutase
dc.subjectNitric oxide
dc.subjectNitric oxide synthase
dc.subjectNADPH oxidase
dc.subjectMedicine
dc.subjectBiochemistry
dc.subjectAmino acid
dc.titleThe restorative effect of apocynin and catalase in l-arginine induced hypotension on normotensive subjects the role of oxidative stressen
dc.typeArticle (peer-reviewed)
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