Interspecific competition between the potato cyst nematode species Globodera pallida and G. rostochiensis

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dc.check.date2021-09-27T10:29:13Z
dc.check.embargoformatE-thesis on CORA onlyen
dc.check.entireThesisEntire Thesis Restricted
dc.check.infoRestricted to everyone for five yearsen
dc.check.opt-outYesen
dc.check.reasonThis thesis is due for publication or the author is actively seeking to publish this materialen
dc.contributor.advisorJones, Peter W.en
dc.contributor.authorHearne, Rachel
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2016-09-28T10:29:13Z
dc.date.issued2016
dc.date.submitted2016
dc.description.abstractThe two potato cyst nematode species, Globodera pallida and G. rostochiensis, are among the most important pests of potato. PCN are difficult to manage, while the two species respond differently to the main control methods. An increase in the incidence of G. pallida had been reported and is generally attributed to greater effectiveness of control measures against G. rostochiensis. The status of PCN in Ireland was studied using PCR. The results demonstrated qPCR to be an efficient means of high-throughput PCN sampling, being able to accurately identify both species in mixed-species populations. Species discrimination using qPCR revealed an increase in the incidence of G. pallida in Ireland in the absence of G. pallida-selective control measures. The population dynamics of G. pallida and G. rostochiensis in Ireland were studied in mixed- and single-species competition assays in vivo. G. pallida proved to be the more successful species, with greater multiplication in mixed- than single-species populations, with G. rostochiensis showing the opposite. This effect was similarly observed in staggered inoculation trials and population proportion trials. It was hypothesised that the greater G. pallida competitiveness could be attributed to its later hatch. G. pallida exhibited a later peak in hatching activity and more prolonged hatch, relative to G. rostochiensis. G. rostochiensis hatch was significantly reduced in mixedspecies hatching assays. G. pallida hatch was significantly higher when hatch was induced in potato root leachates containing G. rostochiensis-specific compounds, indicating that G. pallida hatch is stimulated upon perception of G. rostochiensis–derived compounds. Rhizotron studies revealed that root damage, caused by feeding of the early-hatching G. rostochiensis, resulted in increased lateral root proliferation and significantly increased G. pallida multiplication. Split-root trials indicated a significant G. pallida-induced ISR effect. G. rostochiensis multiplication was significantly reduced in split-root rhizotrons when G. pallida colonised roots before or after G. rostochiensis infection.en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHearne, R. 2016. Interspecific competition between the potato cyst nematode species Globodera pallida and G. rostochiensis. PhD Thesis, University College Cork.en
dc.identifier.endpage243en
dc.identifier.urihttps://hdl.handle.net/10468/3125
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2016, Rachel Hearne.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectPotato cyst nematodeen
dc.subjectGlobodera rostochiensisen
dc.subjectGlobodera pallidaen
dc.subjectInterspecific competitionen
dc.thesis.opt-outtrue
dc.titleInterspecific competition between the potato cyst nematode species Globodera pallida and G. rostochiensisen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Science)en
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