Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells

dc.contributor.authorRahme, Kamil
dc.contributor.authorMinassian, G.
dc.contributor.authorGhanem, Esther
dc.contributor.authorSouaid, Eddy
dc.contributor.authorGuo, Jianfeng
dc.contributor.authorO'Driscoll, Caitríona M.
dc.contributor.authorHolmes, Justin D.
dc.contributor.funderNational Council for Scientific Researchen
dc.date.accessioned2019-12-05T09:45:18Z
dc.date.available2019-12-05T09:45:18Z
dc.date.issued2018-05
dc.date.updated2019-12-05T09:37:34Z
dc.description.abstractHerein we describe a simple method for the synthesis of dendrigraft poly-L-lysine (d-PLL) coated different sizes gold nanoparticles in water (AuNPs-d-PLL) as potential to delivery vehicles of siRNA to PC-3 prostate cancer cells. AuNPs-d-PLL with diameters ranging between 50-120 nm have been synthesised in aqueous solutions using d-PLL (7 KD) as a capping ligand, and ascorbic acid as a reducing agent. The size of the resulting AuNPs was found to depend on several parameters (i.e. the concentrations of the gold salt, ascorbic d-PLL and temperature). The obtained AuNPs-d-PLL were characterized using UV-visible spectroscopy (UV-vis), Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability to PEGylate the AuNPs-d-PLL with SH-PEG-OCH3 and SH-PEG-Folate was demonstrated via DLS and zeta potential mesurements, their capacity to complex siRNA was verified (DLS, gel electrophoresis), and transfer of AuNPs-d-PLL-PEG-FA.siRNA to PC-3 cells was investigated.en
dc.description.sponsorshipNational Council for Scientific Research Lebanon (CNRS-L-GRP2015-3538)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRahme, K., Minassian, G., Ghanem, E., Souaid, E., Guo, J., O'Driscoll, C. M., and Holmes, J. D. (2018) 'Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells', 11th Annual TechConnect World Innovation Conference and Expo, Anaheim, CA. USA, 13-16 May, TechConnect Briefs, pp. 91-94. isbn: 978-0-9975117-8-9en
dc.identifier.endpage94en
dc.identifier.isbn978-0-9975117-8-9
dc.identifier.startpage91en
dc.identifier.urihttps://hdl.handle.net/10468/9332
dc.language.isoenen
dc.publisherTechConnecten
dc.rights© 2018 TechConnect. All rights reserveden
dc.subjectCanceren
dc.subjectGold nanoparticlesen
dc.subjectPolymersen
dc.subjectsiRNA deliveryen
dc.subjectStabilizationen
dc.subjectPhase locked loopsen
dc.subjectAmino acidsen
dc.subjectAscorbic aciden
dc.subjectCellsen
dc.subjectDiseasesen
dc.subjectDynamic light scatteringen
dc.subjectElectrophoresisen
dc.subjectFiber optic sensorsen
dc.subjectMetal nanoparticlesen
dc.subjectRNAen
dc.subjectScanning electron microscopyen
dc.subjectSolutionsen
dc.subjectStabilizationen
dc.subjectSynthesis (chemical)en
dc.subjectUltraviolet visible spectroscopyen
dc.subjectUrologyen
dc.titleDendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cellsen
dc.typeConference itemen
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