Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells
dc.contributor.author | Rahme, Kamil | |
dc.contributor.author | Minassian, G. | |
dc.contributor.author | Ghanem, Esther | |
dc.contributor.author | Souaid, Eddy | |
dc.contributor.author | Guo, Jianfeng | |
dc.contributor.author | O'Driscoll, Caitríona M. | |
dc.contributor.author | Holmes, Justin D. | |
dc.contributor.funder | National Council for Scientific Research | en |
dc.date.accessioned | 2019-12-05T09:45:18Z | |
dc.date.available | 2019-12-05T09:45:18Z | |
dc.date.issued | 2018-05 | |
dc.date.updated | 2019-12-05T09:37:34Z | |
dc.description.abstract | Herein we describe a simple method for the synthesis of dendrigraft poly-L-lysine (d-PLL) coated different sizes gold nanoparticles in water (AuNPs-d-PLL) as potential to delivery vehicles of siRNA to PC-3 prostate cancer cells. AuNPs-d-PLL with diameters ranging between 50-120 nm have been synthesised in aqueous solutions using d-PLL (7 KD) as a capping ligand, and ascorbic acid as a reducing agent. The size of the resulting AuNPs was found to depend on several parameters (i.e. the concentrations of the gold salt, ascorbic d-PLL and temperature). The obtained AuNPs-d-PLL were characterized using UV-visible spectroscopy (UV-vis), Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability to PEGylate the AuNPs-d-PLL with SH-PEG-OCH3 and SH-PEG-Folate was demonstrated via DLS and zeta potential mesurements, their capacity to complex siRNA was verified (DLS, gel electrophoresis), and transfer of AuNPs-d-PLL-PEG-FA.siRNA to PC-3 cells was investigated. | en |
dc.description.sponsorship | National Council for Scientific Research Lebanon (CNRS-L-GRP2015-3538) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | Rahme, K., Minassian, G., Ghanem, E., Souaid, E., Guo, J., O'Driscoll, C. M., and Holmes, J. D. (2018) 'Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells', 11th Annual TechConnect World Innovation Conference and Expo, Anaheim, CA. USA, 13-16 May, TechConnect Briefs, pp. 91-94. isbn: 978-0-9975117-8-9 | en |
dc.identifier.endpage | 94 | en |
dc.identifier.isbn | 978-0-9975117-8-9 | |
dc.identifier.startpage | 91 | en |
dc.identifier.uri | https://hdl.handle.net/10468/9332 | |
dc.language.iso | en | en |
dc.publisher | TechConnect | en |
dc.rights | © 2018 TechConnect. All rights reserved | en |
dc.subject | Cancer | en |
dc.subject | Gold nanoparticles | en |
dc.subject | Polymers | en |
dc.subject | siRNA delivery | en |
dc.subject | Stabilization | en |
dc.subject | Phase locked loops | en |
dc.subject | Amino acids | en |
dc.subject | Ascorbic acid | en |
dc.subject | Cells | en |
dc.subject | Diseases | en |
dc.subject | Dynamic light scattering | en |
dc.subject | Electrophoresis | en |
dc.subject | Fiber optic sensors | en |
dc.subject | Metal nanoparticles | en |
dc.subject | RNA | en |
dc.subject | Scanning electron microscopy | en |
dc.subject | Solutions | en |
dc.subject | Stabilization | en |
dc.subject | Synthesis (chemical) | en |
dc.subject | Ultraviolet visible spectroscopy | en |
dc.subject | Urology | en |
dc.title | Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells | en |
dc.type | Conference item | en |