The use of high performance liquid chromatography for the characterization of the unfolding and aggregation of dairy proteins

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dc.contributor.authorGaspard, Sophie J.
dc.contributor.authorBrodkorb, André
dc.contributor.editorMcManus, Jennifer J.
dc.contributor.funderDairy Research Irelanden
dc.contributor.funderFood Institutional Research Measureen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderEnterprise Irelanden
dc.contributor.funderTeagascen
dc.date.accessioned2019-09-02T15:18:20Z
dc.date.available2019-09-02T15:18:20Z
dc.date.issued2019-07-25
dc.description.abstractHigh-performance liquid chromatography (HPLC) is routinely used to identify and characterize proteins. HPLC can help to understand protein aggregation processes in dairy products, which are induced by common industrial processing steps such as heat treatment. In this chapter, three complementary chromatographic methods are described, which are based on the principles of size exclusion and reversed-phase chromatography. These methods are used to determine the degree of denaturation and aggregation of proteins, and estimate the molecular weight of these aggregates.en
dc.description.sponsorshipDairy Research Ireland (Dairy Levy Research Trust (project MDDT6261 “ProPart”)); Department of Agriculture, Food and the Marine (Food Institutional Research Measure (FIRM, project no. 08RDTMFRC650)); Enterprise Ireland (Food for Health Ireland Project (Grant Number CC20080001)); Teagasc (Teagasc Walsh Fellowship Scheme (reference number 2012211)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGaspard, S. J. and Brodkorb, A. (2019) 'The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins', in McManus, J.J. (ed.) Protein Self-Assembly: Methods and Protocols. New York, NY: Springer New York, pp. 103-115. doi: 10.1007/978-1-4939-9678-0_8en
dc.identifier.doi10.1007/978-1-4939-9678-0_8en
dc.identifier.endpage115en
dc.identifier.isbn978-1-4939-9677-3
dc.identifier.issn1064-3745
dc.identifier.journaltitleProtein Self-Assembly: Methods and Protocols; Part of the Methods in Molecular Biology book series (MIMB)en
dc.identifier.startpage103en
dc.identifier.urihttps://hdl.handle.net/10468/8436
dc.identifier.volume2039en
dc.language.isoenen
dc.publisherHumana Press; Springer Protocolsen
dc.relation.urihttps://link.springer.com/protocol/10.1007%2F978-1-4939-9678-0_8
dc.rights© Springer Science+Business Media, LLC, part of Springer Nature 2019. This is a post-peer-review, pre-copyedit version of a chapter published in Protein Self-Assembly. Methods in Molecular Biology. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-9678-0_8en
dc.subjectHPLCen
dc.subjectProteinsen
dc.subjectDenaturationen
dc.subjectAggregationen
dc.subjectReversed-phase chromatographyen
dc.subjectSize exclusion chromatographyen
dc.subjectGlobular proteinsen
dc.subjectProtein unfoldingen
dc.subjectMesophasesen
dc.subjectMicroscopyen
dc.subjectCapillary electrophoresisen
dc.titleThe use of high performance liquid chromatography for the characterization of the unfolding and aggregation of dairy proteinsen
dc.typeArticle (peer-reviewed)en
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