A quest of great importance-developing a broad spectrum Escherichia coli phage collection

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dc.contributor.authorCasey, Eoghan
dc.contributor.authorNeve, Horst
dc.contributor.authorFranz, Charles M. A. P.
dc.contributor.authorNoben, Jean-Paul
dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorVentura, Marco
dc.contributor.authorSinderen, Douwe van
dc.contributor.authorMahony, Jennifer
dc.contributor.funderBill and Melinda Gates Foundationen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-10-23T04:10:41Z
dc.date.available2019-10-23T04:10:41Z
dc.date.issued2019-09-26
dc.description.abstractShigella ssp. and enterotoxigenic Escherichia coli are the most common etiological agents of diarrheal diseases in malnourished children under five years of age in developing countries. The ever-growing issue of antibiotic resistance and the potential negative impact of antibiotic use on infant commensal microbiota are significant challenges to current therapeutic approaches. Bacteriophages (or phages) represent an alternative treatment that can be used to treat specific bacterial infections. In the present study, we screened water samples from both environmental and industrial sources for phages capable of infecting E. coli laboratory strains within our collection. Nineteen phages were isolatedand tested for their ability to infect strains within the ECOR collection and E. coli O157:H7 Δstx. Furthermore, since coliphages have been reported to cross-infect certain Shigella spp., we also evaluated the ability of the nineteen phages to infect a representative Shigella sonnei strain from our collection. Based on having distinct (although overlapping in some cases) host ranges, ten phage isolates were selected for genome sequence and morphological characterization. Together, these ten selected phages were shown to infect most of the ECOR library, with 61 of the 72 strains infected by at least one phage from our collection. Genome analysis of the ten phages allowed classification into five previously described genetic subgroups plus one previously underrepresented subgroup.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid899en
dc.identifier.citationKaczorowska, J., Casey, E., Neve, H., Franz, C. M. A. P., Noben, J.-P., Lugli, G. A., Ventura, M., Sinderen, D. v. and Mahony, J. (2019) 'A Quest of Great Importance-Developing a Broad Spectrum Escherichia coli Phage Collection', Viruses, 11(10), 899. (19pp.) DOI: 10.3390/v11100899en
dc.identifier.doi10.3390/10.3390/v11100899en
dc.identifier.eissn1999-4915
dc.identifier.endpage19en
dc.identifier.issued10en
dc.identifier.journaltitleVirusesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8825
dc.identifier.volume11en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.relation.urihttps://www.mdpi.com/1999-4915/11/10/899/htm
dc.rights©2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophageen
dc.subjectEscherichia colien
dc.subjectHost rangeen
dc.subjectPhage therapyen
dc.subjectShigella sspen
dc.titleA quest of great importance-developing a broad spectrum Escherichia coli phage collectionen
dc.typeArticle (peer-reviewed)en
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