Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes

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dc.contributor.authorDalfen, Irene
dc.contributor.authorDmitriev, Ruslan I.
dc.contributor.authorHolst, Gerhard
dc.contributor.authorKlimant, Ingo
dc.contributor.authorBorisov, Sergey M.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2018-12-18T11:14:29Z
dc.date.available2018-12-18T11:14:29Z
dc.date.issued2018-12-06
dc.date.updated2018-12-13T12:42:59Z
dc.description.abstractNovel fluorescent diazaoxatriangulenium (DAOTA) pH indicators for lifetime-based self-referenced pH sensing are reported. The DAOTA dyes were decorated with phenolic receptor groups inducing fluorescence quenching via photoinduced electron transfer mechanism. Electron-withdrawing chlorine substituents ensure response in the most relevant pH range (apparent pK'a values ~5 and 7.5 for the p,p-dichlorophenol- and the p-chlorophenol-substituted dyes, respectively). The dyes feature long fluorescence lifetime (17-20 ns), high quantum yield (~60%) and high photostability. Planar optodes are prepared upon immobilization of the dyes into polyurethane hydrogel D4. Apart from the response in the fluorescence intensity, the optodes show pH-dependent lifetime behaviour which makes them suitable for studying 2D pH distribution with help of fluorescence lifetime imaging technique. The lifetime response is particularly pronounced for the sensors with high dye concentration (0.5-1% wt. in respect to the polymer) and is attributed to efficient homo-FRET mechanism.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDalfen, I., Dmitriev, R. I., Holst, G., Klimant, I.and Borisov, S. M. (2018) 'Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes', Analytical Chemistry. doi:10.1021/acs.analchem.8b02534en
dc.identifier.doi10.1021/acs.analchem.8b02534
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.journaltitleAnalytical Chemistryen
dc.identifier.urihttps://hdl.handle.net/10468/7237
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/13/SIRG/2144/IE/Development of Bionic Sensor Materials for Metabolic Imaging in Regenerative Medicine/en
dc.rights© 2018, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry after technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.analchem.8b02534en
dc.subjectDecay timeen
dc.subjectFRETen
dc.subjectpH sensoren
dc.subjectTrianguleniumen
dc.subjectPhotoinduced electron transferen
dc.subjectFrequency Domain FLIMen
dc.titleBackground-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyesen
dc.typeArticle (peer-reviewed)en
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