Neutral red retention time assay in determination of toxicity of nanoparticles

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dc.check.date2017-05-16
dc.check.infoAccess to this article is restricted until 24 months after publication by the request of the publisher.en
dc.contributor.authorHu, Wentao
dc.contributor.authorCulloty, Sarah C.
dc.contributor.authorDarmody, Grainne
dc.contributor.authorLynch, Sharon A.
dc.contributor.authorDavenport, John
dc.contributor.authorRamírez-García, Sonia
dc.contributor.authorDawson, Kenneth
dc.contributor.authorLynch, Iseult
dc.contributor.authorDoyle, Hugh
dc.contributor.authorSheehan, David
dc.date.accessioned2016-04-19T08:36:44Z
dc.date.available2016-04-19T08:36:44Z
dc.date.issued2015-05-16
dc.date.updated2016-04-10T17:49:16Z
dc.description.abstractThe neutral red retention time (NRRT) assay is useful for detecting decreased lysosomal membrane stability in haemocytes sampled from bivalves, a phenomenon often associated with exposure to environmental pollutants including nanomaterials. Bivalves are popular sentinel species in ecotoxicology and use of NRRT in study of species in the genus Mytilus is widespread in environmental monitoring. The NRRT assay has been used as an in vivo test for toxicity of carbon nanoparticles (Moore MN, Readman JAJ, Readman JW, Lowe DM, Frickers PE, Beesley A. 2009. Lysosomal cytotoxicity of carbon nanoparticles in cells of the molluscan immune system: An in vivo study. Nanotoxicology. 3 (1), 40-45). We here report application of this assay adapted to a microtitre plate format to a panel of metal and metal oxide nanoparticles (2 ppm). This showed that copper, chromium and cobalt nanoparticles are toxic by this criterion while gold and titanium nanoparticles are not. As the former three nanoparticles are often reported to be cytotoxic while the latter two are thought to be non-cytotoxic, these data support use of NRRT as a general in vitro assay in nanotoxicology.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHU, W., CULLOTY, S., DARMODY, G., LYNCH, S., DAVENPORT, J., RAMIREZ-GARCIA, S., DAWSON, K., LYNCH, I., DOYLE, H. & SHEEHAN, D. 2015. Neutral red retention time assay in determination of toxicity of nanoparticles. Marine Environmental Research, 111, 158-161. doi:10.1016/j.marenvres.2015.05.007en
dc.identifier.doi10.1016/j.marenvres.2015.05.007
dc.identifier.endpage161en
dc.identifier.issn0141-1136
dc.identifier.journaltitleMarine Environmental Researchen
dc.identifier.startpage158en
dc.identifier.urihttps://hdl.handle.net/10468/2456
dc.identifier.volume111en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.relation.urihttp://dx.doi.org/10.1016/j.marenvres.2015.05.007
dc.rightsCopyright © 2015 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectMytilusen
dc.subjectMetal oxideen
dc.subjectLysosomeen
dc.subjectMembrane stabilityen
dc.titleNeutral red retention time assay in determination of toxicity of nanoparticlesen
dc.typeArticle (peer-reviewed)en
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