Bifidobacterial genes upregulated by resistant starch investigated using multi-omics have orthologs in infant gut isolates

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dc.contributor.authorMillar, Molly E.
dc.contributor.authorAbele, Miriam
dc.contributor.authorHarris, Hannah C.
dc.contributor.authorKoev, Todor T.
dc.contributor.authorTelatin, Andrea
dc.contributor.authorKiu, Raymond
dc.contributor.authorVan Sinderen, Douwe
dc.contributor.authorKhimyak, Yaroslav Z.
dc.contributor.authorLudwig, Christina
dc.contributor.authorHall, Lindsay J.
dc.contributor.authorWarren, Frederick J.
dc.contributor.funderUKRI Biotechnology and Biological Sciences Research Council
dc.date.accessioned2026-09-09T11:55:01Z
dc.date.available2026-09-09T11:55:01Z
dc.date.issued2026-05-17
dc.description.abstractBifidobacterium species and strains are key members of the human gut microbiota, appearing soon after birth and persisting into adulthood. Resistant starch is an important dietary substrate for adult-associated bifidobacteria, where its fermentation supports host health. However, less is known about how different starch structures interact with bifidobacteria. Here we show that growth kinetics and gene expression differ depending on starch structure. Using detailed growth assays, genomics, and metabolomic analyses, bifidobacterial starch hydrolysis capabilities were closely associated with their CAZyme profiles. In one isolate of Bifidobacterium globosum, we identified a gene cluster encoding three multi-functional amylase enzymes complemented by several starch-binding modules, the genes and proteins of which were significantly upregulated in response to starch. Homologs of genes in the cluster were found in the genomes of bifidobacterial isolates from weaning infants providing insights into their role in the maturation process of the microbiota. Uncovering mechanisms of metabolic interaction between starch structures and bifidobacteria underscores the importance of this ecological function and potential health implications.en
dc.description.versionPublished Version
dc.format.extent13
dc.format.mimetypeapplication/pdfen
dc.identifier.articleidycag136
dc.identifier.authororcidMillar, Molly E.
dc.identifier.authororcidAbele, Miriam
dc.identifier.authororcidHarris, Hannah C.
dc.identifier.authororcidKoev, Todor T.
dc.identifier.authororcidTelatin, Andrea
dc.identifier.authororcidKiu, Raymond
dc.identifier.authororcidVan Sinderen, Douwe§0000-0003-1823-7957
dc.identifier.authororcidKhimyak, Yaroslav Z.
dc.identifier.authororcidLudwig, Christina
dc.identifier.authororcidHall, Lindsay J.
dc.identifier.authororcidWarren, Frederick J.
dc.identifier.citationMillar, M E, Abele, M, Harris, H C, Koev, T T, Telatin, A, Kiu, R, Van Sinderen, D, Khimyak, Y Z, Ludwig, C, Hall, L J & Warren, F J 2026, 'Bifidobacterial genes upregulated by resistant starch investigated using multi-omics have orthologs in infant gut isolates', ISME Communications, vol. 6, no. 1, ycag136, pp. 1-13. https://doi.org/10.1093/ismeco/ycag136
dc.identifier.doi10.1093/ismeco/ycag136
dc.identifier.endpage13
dc.identifier.issn2730-6151
dc.identifier.issued1
dc.identifier.journaltitleISME Communications
dc.identifier.otherORCID: /0000-0003-1823-7957/work/226277162
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/19209
dc.identifier.volume6
dc.language.isoen
dc.publisherOxford University Press
dc.relation.urihttps://www.scopus.com/pages/publications/105042111359
dc.rights© 2026, the Author(s). Published by Oxford University Press on behalf of the International Society for Microbial Ecology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accessrightsopen access
dc.rights.licensenameAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.statusPeer reviewed
dc.subjectBifidobacteria
dc.subjectCAZymes
dc.subjectMicrobiome
dc.subjectResistant starch
dc.subject[Microbiology]
dc.subject[APCMicrobiome]
dc.titleBifidobacterial genes upregulated by resistant starch investigated using multi-omics have orthologs in infant gut isolatesen
dc.typeArticle (peer-reviewed)
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