The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

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dc.contributor.authorClamens, Thomas
dc.contributor.authorRosay, Thibaut
dc.contributor.authorCrépin, Alexandre
dc.contributor.authorGrandjean, Teddy
dc.contributor.authorKentache, Takfarinas
dc.contributor.authorHardouin, Julie
dc.contributor.authorBortolotti, Perrine
dc.contributor.authorNeidig, Anke
dc.contributor.authorMooij, Marlies J.
dc.contributor.authorHillion, Mélanie
dc.contributor.authorVieillard, Julien
dc.contributor.authorCosette, Pascal
dc.contributor.authorOverhage, Joerg
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorBouffartigues, Emeline
dc.contributor.authorDufour, Alain
dc.contributor.authorChevalier, Sylvie
dc.contributor.authorGuery, Benoit
dc.contributor.authorCornelis, Pierre
dc.contributor.authorFeuilloley, Marc G. J.
dc.contributor.authorLesouhaitier, Olivier
dc.contributor.funderMinistère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
dc.contributor.funderEuropean Regional Development Fund
dc.contributor.funderAssociation Vaincre la Mucoviscidose
dc.contributor.funderMinistère des Affaires Étrangères
dc.contributor.funderKarlsruher Institut für Technologie
dc.date.accessioned2017-02-03T09:56:54Z
dc.date.available2017-02-03T09:56:54Z
dc.date.issued2017-01-24
dc.date.updated2017-02-03T09:41:44Z
dc.description.abstractWe have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE+) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE+ strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.en
dc.description.sponsorshipMinistère des Affaires Étrangères (Partenariats Hubert Curien (PHC Ulysses)); Karlsruher Institut für Technologie (BioInterfaces (BIF) Program of the Karlsruhe Institute of Technology (KIT) in the Helmholtz Association)
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationClamens, T., Rosay, T., Crépin, A., Grandjean, T., Kentache, T., Hardouin, J., Bortolotti, P., Neidig, A., Mooij, M., Hillion, M., Vieillard, J., Cosette, P., Overhage, J., O’Gara, F., Bouffartigues, E., Dufour, A., Chevalier, S., Guery, B., Cornelis, P., Feuilloley, M. G. J. and Lesouhaitier, O. (2017) 'The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence', Scientific Reports, 7, pp. 41178. doi:10.1038/srep41178en
dc.identifier.doi10.1038/srep41178
dc.identifier.endpage41178-16en
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage41178-1en
dc.identifier.urihttps://hdl.handle.net/10468/3559
dc.identifier.volume7en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriologyen
dc.subjectMicrobiologyen
dc.subjectPseudomonas aeruginosa virulenceen
dc.subjectVirulence regulationen
dc.subjectAmiE overproductionen
dc.titleThe aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulenceen
dc.typeArticle (peer-reviewed)en
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