Metagenomic approaches for the discovery of pollutant-remediating enzymes: Recent trends and challenges

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dc.check.date2024-09-27en
dc.check.infoAccess to this article is restricted until 24 months after publication by request of the publisher.en
dc.contributor.authorMukherjee, Arghyaen
dc.contributor.authorCotter, Paul D.en
dc.date.accessioned2023-06-22T14:11:31Z
dc.date.available2023-06-22T14:11:31Z
dc.date.issued2022-09-27en
dc.description.abstractMetagenomic studies in diverse environments have generated petabytes of sequencing data, allowing biologists to peer into the uncultivated microbial majority with unprecedented clarity. Such advances have heralded a new era of enzyme discovery where proteins of interest can be directly extracted from metagenomic sequencing data, although this remains a challenging task. Traditionally, metagenomic enzyme discovery, including that involved in xenobiotic degradation, has largely relied on functional insights derived from activity-guided or PCR-based metagenomics. Due to its untargeted and holistic nature, metagenomics allows us to probe the unknown and underexplored microbial diversity, which represents a key resource for novel biocatalyst discovery. Metagenomic shotgun sequencing-based enzyme discovery, additionally, avoids common biases introduced through PCR-based or activity-guided functional genomic methods. In this chapter, we have provided an overview of metagenomics in novel enzyme discovery with discussions on both the experimental and computational aspects of the same. We discuss in detail computational strategies for identifying possible enzyme candidates from shotgun sequencing data and experimental strategies for characterizing candidate enzymes once they have been identified. Finally, we review emerging methods of metagenomic enzyme discovery as well as future goals and challenges with an emphasis on metagenomic-based approaches.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMukherjee, A. and Cotter, P. D. (2022) 'Metagenomic approaches for the discovery of pollutant-remediating enzymes: Recent trends and challenges', in Kumar, V. and Thakur, I. S. (eds) Omics Insights in Environmental Bioremediation. Springer, Singapore, pp. 571-604. doi: 10.1007/978-981-19-4320-1_24en
dc.identifier.doi10.1007/978-981-19-4320-1_24en
dc.identifier.endpage604en
dc.identifier.isbn9789811943195en
dc.identifier.isbn9789811943201en
dc.identifier.startpage571en
dc.identifier.urihttps://hdl.handle.net/10468/14687
dc.language.isoenen
dc.publisherSpringer Nature Ltd.en
dc.relation.ispartofOmics Insights in Environmental Bioremediationen
dc.rights© 2022, Springer Nature Singapore Pte Ltd. This is a post-peer-review, pre-copyedit version of a chapter published in Kumar, V. and Thakur, I. S. (eds) Omics Insights in Environmental Bioremediation. Springer, Singapore, pp. 571-604, doi: 10.1007/978-981-19-4320-1_24. The final authenticated version is available online at: https://doi.org/10.1007/978-981-19-4320-1_24en
dc.subjectMetagenomicsen
dc.subjectXenobioticsen
dc.subjectPollutionen
dc.subjectEnzyme discoveryen
dc.subjectShotgun sequencingen
dc.subjectProtein functionen
dc.titleMetagenomic approaches for the discovery of pollutant-remediating enzymes: Recent trends and challengesen
dc.typeBook chapteren
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