dc.contributor.author	Lango-Scholey, Lea
dc.contributor.author	Brachmann, Alexander O.
dc.contributor.author	Bode, Helge B.
dc.contributor.author	Clarke, David J.
dc.contributor.funder	Science Foundation Ireland
dc.date.accessioned	2016-02-17T11:44:42Z
dc.date.available	2016-02-17T11:44:42Z
dc.date.issued	2013
dc.description.abstract	Photorhabdus is a genus of Gram-negative entomopathogenic bacteria that also maintain a mutualistic association with nematodes from the family Heterorhabditis. Photorhabdus has an extensive secondary metabolism that is required for the interaction between the bacteria and the nematode. A major component of this secondary metabolism is a stilbene molecule, called ST. The first step in ST biosynthesis is the non-oxidative deamination of phenylalanine resulting in the production of cinnamic acid. This reaction is catalyzed by phenylalanine-ammonium lyase, an enzyme encoded by the stlA gene. In this study we show, using a stlA-gfp transcriptional fusion, that the expression of stlA is regulated by nutrient limitation through a regulatory network that involves at least 3 regulators. We show that TyrR, a LysR-type transcriptional regulator that regulates gene expression in response to aromatic amino acids in E. coli, is absolutely required for stlA expression. We also show that stlA expression is modulated by σS and Lrp, regulators that are implicated in the regulation of the response to nutrient limitation in other bacteria. This work is the first that describes pathway-specific regulation of secondary metabolism in Photorhabdus and, therefore, our study provides an initial insight into the complex regulatory network that controls secondary metabolism, and therefore mutualism, in this model organism.	en
dc.description.sponsorship	Science Foundation Ireland (Research Frontiers Programme RFP/07/GENF546)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	UNSP e82152
dc.identifier.citation	Lango-Scholey L, Brachmann AO, Bode HB, Clarke DJ (2013) The Expression of stlA in Photorhabdus luminescens Is Controlled by Nutrient Limitation. PLoS ONE 8(11): e82152. doi:10.1371/journal.pone.0082152
dc.identifier.doi	10.1371/journal.pone.0082152
dc.identifier.issn	1932-6203
dc.identifier.issued	11	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2354
dc.identifier.volume	8	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2015 Lango-Scholey et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Green fluorescent protein	en
dc.subject	General stress response	en
dc.subject	Escherichia coli	en
dc.subject	Antibiotic biosynthesis	en
dc.subject	Streptomyces coelicolor	en
dc.subject	Phenotypic variation	en
dc.subject	Regulatory proteins	en
dc.subject	Nematode symbiont	en
dc.subject	Stationary-phase	en
dc.subject	Metabolic switch	en
dc.title	The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation	en
dc.type	Article (peer-reviewed)	en

The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation

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