Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: a redox proteomic investigation
| dc.check.info | Access to this article is restricted until 24 months after publication by request of the publisher. | en |
| dc.contributor.author | Bouallegui, Younes | |
| dc.contributor.author | Ben Younes, Ridha | |
| dc.contributor.author | Oueslati, Ridha | |
| dc.contributor.author | Sheehan, David | |
| dc.contributor.funder | Ministry of Higher Education and Scientific Research, Tunisia | |
| dc.date.accessioned | 2018-05-15T10:43:59Z | |
| dc.date.available | 2018-05-15T10:43:59Z | |
| dc.date.issued | 2018-04-23 | |
| dc.date.updated | 2018-05-01T08:53:04Z | |
| dc.description.abstract | Oxidative stress is often implicated in nanoparticle toxicity. Several studies have highlighted the role of internalization routes in determining nanotoxicity. Here, we investigate how two endocytotic mechanisms (clathrin- and caveolae-mediated) impact on redox balance in gill and digestive gland of the mussel, Mytilus galloprovincialis. Animals were exposed (for 3, 6 and 12 h) to two sizes of silver nanoparticles (AgNP: <50 nm and <100 nm) prior to and after blockade of two endocytic pathways (amantadine blocks clathrin-mediated endocytosis while nystatin blocks caveolae-mediated endocytosis). Redox-proteomic tools were used to determine effects. Our results demonstrate the ability of both sizes of AgNP (<50 and <100 nm) to cause protein thiol oxidation and/or protein carbonylation. However, blockade of endocytotic routes mitigated AgNP toxicity. Differential ROS-related toxicity of AgNP to mussel tissues seemed to be linked to tissue-specific mode of action requirements. Cell uptake mechanism strongly influences toxicity of AgNPs in this filter-feeder. | en |
| dc.description.status | Peer reviewed | en |
| dc.description.version | Accepted Version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Bouallegui, Y., Ben Younes, R., Oueslati, R. and Sheehan, D. (2018) 'Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: a redox proteomic investigation', Aquatic Toxicology. doi: 10.1016/j.aquatox.2018.04.013 | en |
| dc.identifier.doi | 10.1016/j.aquatox.2018.04.013 | |
| dc.identifier.issn | 0166-445X | |
| dc.identifier.journaltitle | Aquatic Toxicology | en |
| dc.identifier.uri | https://hdl.handle.net/10468/6103 | |
| dc.language.iso | en | en |
| dc.publisher | Elsevier B.V. | en |
| dc.rights | © 2018, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. | en |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
| dc.subject | Oxidative stress | en |
| dc.subject | Carbonyls | en |
| dc.subject | Thiols | en |
| dc.subject | AgNPs | en |
| dc.subject | Endocytosis | en |
| dc.subject | Mussel | en |
| dc.title | Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: a redox proteomic investigation | en |
| dc.type | Article (peer-reviewed) | en |
