Oriented antibody immobilization vs random covalent binding: improving reproducibility for label-free electrochemical immunosensing of multi-allergens on silicon chips

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dc.contributor.authorBozorgzadeh, Somayyehen
dc.contributor.authorJuska, Vuslat B.en
dc.contributor.authorO'Riordan, Alanen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2025-10-08T10:42:01Z
dc.date.available2025-10-08T10:42:01Z
dc.date.issued2025-09-04en
dc.description.abstractThe development of compact biosensing devices capable of sensitive and selective concomitant detection of two or more analytes in a single sample has enormous potential for point-of-care and -use applications. Silicon microfabrication technologies outperform other fabrication processes in terms of reproducibility, miniaturization capability, and design adjustability. In this work, we present a novel miniaturized fully integrated multiplexed silicon-based sensing platform for rapid and label-free detection of milk and egg allergens in water. After modifying the microelectrodes with gold nanostructures and chitosan-gold nanocomposite hydrogel layer, we compared random covalent binding with boronic acid-assisted oriented strategy for antibody immobilization to the surface. The boronic affinity-based approach exhibited a high reproducible and reliable immuno-response towards casein as a major milk allergen. The stepwise surface modifications were characterized using various analytical techniques, confirming the successful attachment of antibodies to the surface. We then successfully adapted the biosensor surface for ovalbumin detection, an egg allergen, demonstrating multiplexed allergen detection and universal immobilization strategy for any IgG type antibodies, opening a wide range of potential applications in portable immunosensing for various target analytes beyond food allergens.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBozorgzadeh, S., Juska, V. B. and O'Riordan, A. (2025) 'Oriented antibody immobilization vs random covalent binding: improving reproducibility for label-free electrochemical immunosensing of multi-allergens on silicon chips', 2025 IEEE Applied Sensing Conference (APSCON), Hyderabad, India, 20-22 January 2025, pp. 315-318. https://doi.org/10.1109/APSCON63569.2025.11144303en
dc.identifier.doi10.1109/apscon63569.2025.11144303en
dc.identifier.endpage318en
dc.identifier.isbn979-8-3503-7933-4en
dc.identifier.isbn979-8-3503-7934-1en
dc.identifier.startpage315en
dc.identifier.urihttps://hdl.handle.net/10468/17986
dc.language.isoenen
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.relation.ispartof2025 IEEE Applied Sensing Conference (APSCON), Hyderabad, India, 20-22 January 2025en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/Research Centres Programme/16/RC/3835/IE/VistaMilk Centre/en
dc.rights© 2024, IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.en
dc.subjectSilicon microfabricationen
dc.subjectMultiplexed electrochemical immunosensingen
dc.subjectLabel free detectionen
dc.subjectFood allergensen
dc.subjectSite-selective orientationsen
dc.titleOriented antibody immobilization vs random covalent binding: improving reproducibility for label-free electrochemical immunosensing of multi-allergens on silicon chipsen
dc.typeConference itemen
dc.typeproceedings-articleen
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