Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice

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dc.contributor.author Cronin, Michelle
dc.contributor.author Sleator, Roy D.
dc.contributor.author Hill, Colin
dc.contributor.author Fitzgerald, Gerald F.
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2010-03-15T12:47:26Z
dc.date.available 2010-03-15T12:47:26Z
dc.date.copyright 2008
dc.date.issued 2008-09-24
dc.identifier.citation Cronin, M., Sleator, R.D., Hill, C., Fitzgerald, G.F., van Sinderen, D., 2008. Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice. BMC Microbiology, 8:161, doi:10.1186/1471-2180-8-161 en
dc.identifier.volume 8 en
dc.identifier.startpage 161 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/121
dc.identifier.doi 10.1186/1471-2180-8-161
dc.description.abstract Background: Probiotics such as bifidobacteria have been shown to maintain a healthy intestinal microbial balance and help protect against infections. However, despite these benefits, bifidobacteria still remain poorly understood at the biochemical, physiological and especially the genetic level. Herein we describe, for the first time, the development of a non-invasive luciferase based reporter system for real-time tracking of Bifidobacterium species in vivo. Results: The reporter vector pLuxMC1 is based on the recently described theta-type plasmid pBC1 from B. catenatulatum [1] and the luxABCDE operon from pPL2lux [2]. Derivatives of pLuxMC1, harbouring a bifidobacterial promoter (pLuxMC2) as well as a synthetically derived promoter (pLuxMC3) [3] placed upstream of luxABCDE, were constructed and found to stably replicate in B. breve UCC2003. The subsequent analysis of these strains allowed us to assess the functionality of pLuxMC1 both in vitro and in vivo. Conclusion: Our results demonstrate the potential of pLuxMC1 as a real-time, non-invasive reporter system for Bifidobacterium. It has also allowed us, for the first time, to track the colonisation potential and persistence of this probiotic species in real time. An interesting and significant outcome of the study is the identification of the caecum as a niche environment for B. breve UCC2003 within the mouse gastrointestinal tract (GI) tract. en
dc.description.sponsorship Science Foundation Ireland (Centre for Science Engineering and Technology) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.relation.uri http://www.biomedcentral.com/1471-2180/8/161
dc.rights © 2008 Cronin et al; licensee BioMed Central Ltd. en
dc.rights.uri http://creativecommons.org/licenses/by/2.0/ en
dc.subject Probiotics en
dc.subject Luciferases en
dc.subject.lcsh Gastrointestinal system -- Bacterial Growth en
dc.subject.lcsh Bifidobacterium en
dc.title Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Michelle Cronin, Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail: m.a.cronin@student.ucc.ie en
dc.internal.authorcontactother Roy D Sleator, Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail: r.sleator@ucc.ie en
dc.internal.authorcontactother Colin Hill, Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail: c.hill@ucc.ie en
dc.internal.authorcontactother Gerald F Fitzgerald, Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail:g.fitz@ucc.ie en
dc.internal.authorcontactother Douwe van Sinderen, Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail:d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.status Peer reviewed en
dc.description.version Published Version en
dc.internal.rssid 40646047
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Health Research Board en
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en


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© 2008 Cronin et al; licensee BioMed Central Ltd. Except where otherwise noted, this item's license is described as © 2008 Cronin et al; licensee BioMed Central Ltd.
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