Investigation of micro-devices for neurobiological applications

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dc.contributor.advisor Galvin, Paul en
dc.contributor.advisor McDermott, Kieran en
dc.contributor.advisor Herzog, Grégoire en
dc.contributor.advisor Arrigan, Damien en
dc.contributor.author Grygoryev, Konstantin
dc.date.accessioned 2013-10-10T15:58:17Z
dc.date.available 2013-10-10T15:58:17Z
dc.date.issued 2013
dc.date.submitted 2013
dc.identifier.citation Grygoryev, K. 2013. Investigation of micro-devices for neurobiological applications. PhD Thesis, University College Cork. en
dc.identifier.endpage 192
dc.identifier.uri http://hdl.handle.net/10468/1249
dc.description.abstract The aim of this project is to integrate neuronal cell culture with commercial or in-house built micro-electrode arrays and MEMS devices. The resulting device is intended to support neuronal cell culture on its surface, expose specific portions of a neuronal population to different environments using microfluidic gradients and stimulate/record neuronal electrical activity using micro-electrode arrays. Additionally, through integration of chemical surface patterning, such device can be used to build neuronal cell networks of specific size, conformation and composition. The design of this device takes inspiration from the nervous system because its development and regeneration are heavily influenced by surface chemistry and fluidic gradients. Hence, this device is intended to be a step forward in neuroscience research because it utilizes similar concepts to those found in nature. The large part of this research revolved around solving technical issues associated with integration of biology, surface chemistry, electrophysiology and microfluidics. Commercially available microelectrode arrays (MEAs) are mechanically and chemically brittle making them unsuitable for certain surface modification and micro-fluidic integration techniques described in the literature. In order to successfully integrate all the aspects into one device, some techniques were heavily modified to ensure that their effects on MEA were minimal. In terms of experimental work, this thesis consists of 3 parts. The first part dealt with characterization and optimization of surface patterning and micro-fluidic perfusion. Through extensive image analysis, the optimal conditions required for micro-contact printing and micro-fluidic perfusion were determined. The second part used a number of optimized techniques and successfully applied these to culturing patterned neural cells on a range of substrates including: Pyrex, cyclo-olefin and SiN coated Pyrex. The second part also described culturing neurons on MEAs and recording electrophysiological activity. The third part of the thesis described integration of MEAs with patterned neuronal culture and microfluidic devices. Although integration of all methodologies proved difficult, a large amount of data relating to biocompatibility, neuronal patterning, electrophysiology and integration was collected. Original solutions were successfully applied to solve a number of issues relating to consistency of micro printing and microfluidic integration leading to successful integration of techniques and device components. en
dc.description.sponsorship Higher Education Authority (PRTLI 4) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2013, Konstantin Grygoryev en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Reversible integration en
dc.subject Microfluidics en
dc.subject Micro-electrode arrays en
dc.subject Neuronal patterning en
dc.subject MEMS en
dc.subject.lcsh Microelectromechanical systems en
dc.subject.lcsh Molecular neurobiology en
dc.subject.lcsh Electrophysiology en
dc.title Investigation of micro-devices for neurobiological applications en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en
dc.check.info No embargo required en
dc.description.version Accepted Version
dc.contributor.funder National Biophotonics and Imaging Platform Ireland en
dc.contributor.funder Higher Education Authority en
dc.description.status Not peer reviewed en
dc.internal.school Anatomy en
dc.internal.school Biosciences Institute en
dc.internal.school Tyndall National Institute en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
ucc.workflow.supervisor paul.galvin@tyndall.ie
dc.internal.conferring Autumn Conferring 2013 en


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© 2013, Konstantin Grygoryev Except where otherwise noted, this item's license is described as © 2013, Konstantin Grygoryev
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