Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna

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dc.contributor.author Rainville, Louis-Charles
dc.contributor.author Carolan, Darragh
dc.contributor.author Varela, Ana Coelho
dc.contributor.author Doyle, Hugh
dc.contributor.author Sheehan, David
dc.date.accessioned 2014-08-27T09:01:21Z
dc.date.available 2015-01-17T05:00:05Z
dc.date.issued 2014-01
dc.identifier.citation RAINVILLE, L.-C., CAROLAN, D., VARELA, A. C., DOYLE, H. & SHEEHAN, D. 2014. Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna. Analyst, 139, 1678-1686. doi: 10.1039/C3AN02160B en
dc.identifier.volume 139 en
dc.identifier.issued 7 en
dc.identifier.startpage 1678 en
dc.identifier.endpage 1686 en
dc.identifier.issn 0003-2654
dc.identifier.uri http://hdl.handle.net/10468/1631
dc.identifier.doi 10.1039/c3an02160b
dc.description.abstract Recent decades have seen a strong increase in the promise and uses of nanotechnology. This is correlated with their growing release in the environment and there is concern that nanomaterials may endanger ecosystems. Silver nanoparticles (AgNPs) have some of the most varied applications, making their release into the environment unavoidable. In order to assess their potential toxicity in aquatic environments, the acute toxicity of citrate-coated AgNPs to Daphnia magna was measured and compared to that of AgNO3. AgNPs were found to be ten times less toxic by mass than silver ions, and most of this toxicity was removed by ultracentrifuging. At the protein level, the two forms of silver had different impacts. Both increased protein thiol content, while only AgNP increased carbonyl levels. In 2DE of samples labelled for carbonyls, no feature was significantly affected by both compounds, indicating different modes of toxicity. Identified proteins showed functional overlap between the two compounds: vitellogenins (vtg) were present in most features identified, indicating their role as a general stress sensor. In addition to vtg, hemoglobin levels were increased by the AgNP exposure while 14-3-3 protein (a regulatory protein) carbonylation levels were reduced by AgNO3. Overall, this study confirms the previously observed lower acute toxicity of AgNPs, while demonstrating that the toxicity of both forms of silver follow somewhat different biologic pathways, potentially leading to different interactions with natural compounds or pollutants in the aquatic environment. en
dc.description.sponsorship Higher Education Authority (PRTLI program (Cycle 3 Nanoscience and Cycle 4 INSPIRE)) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher The Royal Society of Chemistry en
dc.relation.uri http://pubs.rsc.org/en/content/articlepdf/2014/an/c3an02160b
dc.rights © The Royal Society of Chemistry 2014. Reproduced by permission of The Royal Society of Chemistry. en
dc.subject Analytical toxicology of nanoparticles en
dc.subject Nanoparticle toxicity en
dc.subject Silver nitrate toxicity en
dc.subject Citrate-coated AgNP en
dc.title Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Hugh Doyle, Tyndall Micronano Electronics, University College Cork, Cork, Ireland. +353-21-490-3000 Email: hugh.doyle@tyndall.ie en
dc.internal.availability Full text available en
dc.date.updated 2014-08-01T15:57:16Z
dc.description.version Accepted Version en
dc.internal.rssid 245222280
dc.contributor.funder Higher Education Authority en
dc.contributor.funder Irish Research Council for Science Engineering and Technology en
dc.contributor.funder Fonds Québécois de la Recherche sur la Nature et les Technologies, Canada fr
dc.description.status Peer reviewed en
dc.identifier.journaltitle Analyst en
dc.internal.copyrightchecked No. CORA Romeo - AV + 12 month embargo en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress hugh.doyle@tyndall.ie en


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