The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation

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dc.contributor.author Santos-Martínez, María José Ose
dc.contributor.author Inkielewicz-Stȩpniak, Iwona
dc.contributor.author Medina, Carlos
dc.contributor.author Rahme, Kamil
dc.contributor.author D'Arcy, Deirdre M.
dc.contributor.author Fox, Daniel J.
dc.contributor.author Holmes, Justin D.
dc.contributor.author Zhang, Hongzhou
dc.contributor.author Radomski, Marek Witold
dc.date.accessioned 2016-02-10T17:14:34Z
dc.date.available 2016-02-10T17:14:34Z
dc.date.issued 2012-01-13
dc.identifier.citation SANTOS-MARTINEZ, M. J., INKIELEWICZ-STEPNIAK, I., MEDINA, C., RAHME, K., D'ARCY, D. M., FOX, D., HOLMES, J. D., ZHANG, H. Z. & RADOMSKI, M. W. 2012. The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation. International Journal of Nanomedicine, 7, 243-255. http://dx.doi.org/10.2147/IJN.S26679 en
dc.identifier.volume 7 en
dc.identifier.startpage 243 en
dc.identifier.endpage 255 en
dc.identifier.issn 1178-2013
dc.identifier.uri http://hdl.handle.net/10468/2273
dc.identifier.doi 10.2147/ijn.s26679
dc.description.abstract Interactions between blood platelets and nanoparticles have both pharmacological and toxicological significance and may lead to platelet activation and aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for the measurement of platelet microaggregation under flow conditions using a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The aim of the current study was to investigate if QCM-D could be used for the measurement of nanoparticle-platelet interactions. Silica, polystyrene, and gold nanoparticles were tested. The interactions were also studied using light aggregometry and flow cytometry, which measured surface abundance of platelet receptors. Platelet activation was imaged using phase contrast and scanning helium ion microscopy. QCM-D was able to measure nanoparticle-induced platelet microaggregation for all nanoparticles tested at concentrations that were undetectable by light aggregometry and flow cytometry. Microaggregates were measured by changes in frequency and dissipation, and the presence of platelets on the sensor surface was confirmed and imaged by phase contrast and scanning helium ion microscopy. en
dc.description.sponsorship Science Foundation Ireland (SFI (PI 05/FE1/B862), (RFP/BMT2781), and SFI grant on the BioNanoInteract Ireland project.) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Dove Press en
dc.relation.uri http://www.dovepress.com/international-journal-of-nanomedicine-journal
dc.rights © 2012 The Authors; publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. en
dc.rights.uri https://creativecommons.org/licenses/by-nc/3.0/ en
dc.subject Platelet aggregation en
dc.subject Nanoparticles en
dc.subject Light aggregometer en
dc.subject Quartz crystal microbalance with dissipation en
dc.subject Scanning helium ion microscopy en
dc.subject Gold nanoparticle en
dc.subject Polystyrene en
dc.subject Silicon dioxide en
dc.subject Thrombocyte receptor en
dc.subject QCM-D en
dc.subject PADGEM protein en
dc.subject Helium en
dc.title The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Justin D. Holmes, Chemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.holmes@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2013-03-07T20:54:15Z
dc.description.version Published Version en
dc.internal.rssid 120084589
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Journal of Nanomedicine en
dc.internal.copyrightchecked No. !!CORA!! Open Access Facility under CC-BY-NC 3.0 en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress j.holmes@ucc.ie en


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© 2012 The Authors; publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © 2012 The Authors; publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
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