dc.contributor.author |
Fouhy, Fiona |
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dc.contributor.author |
Ogilvie, Lesley A. |
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dc.contributor.author |
Jones, Brian V. |
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dc.contributor.author |
Ross, R. Paul |
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dc.contributor.author |
Ryan, C. Anthony |
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dc.contributor.author |
Dempsey, Eugene M. |
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dc.contributor.author |
Fitzgerald, Gerald F. |
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dc.contributor.author |
Stanton, Catherine |
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dc.contributor.author |
Cotter, Paul D. |
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dc.date.accessioned |
2016-02-17T11:43:39Z |
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dc.date.available |
2016-02-17T11:43:39Z |
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dc.date.issued |
2014 |
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dc.identifier.citation |
Fouhy F, Ogilvie LA, Jones BV, Ross RP, Ryan AC, Dempsey EM, et al. (2014) Identification of Aminoglycoside and β-Lactam Resistance Genes from within an Infant Gut Functional Metagenomic Library. PLoS ONE 9(9): e108016. doi:10.1371/journal.pone.0108016 |
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dc.identifier.volume |
9 |
en |
dc.identifier.issued |
9 |
en |
dc.identifier.issn |
1932-6203 |
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dc.identifier.uri |
http://hdl.handle.net/10468/2326 |
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dc.identifier.doi |
10.1371/journal.pone.0108016 |
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dc.description.abstract |
The infant gut microbiota develops rapidly during the first 2 years of life, acquiring microorganisms from diverse sources. During this time, significant opportunities exist for the infant to acquire antibiotic resistant bacteria, which can become established and constitute the infant gut resistome. With increased antibiotic resistance limiting our ability to treat bacterial infections, investigations into resistance reservoirs are highly pertinent. This study aimed to explore the nascent resistome in antibiotically-naive infant gut microbiomes, using a combination of metagenomic approaches. Faecal samples from 22 six-month-old infants without previous antibiotic exposure were used to construct a pooled metagenomic library, which was functionally screened for ampicillin and gentamicin resistance. Our library of similar to 220Mb contained 0.45 ampicillin resistant hits/Mb and 0.059 gentamicin resistant hits/Mb. PCR-based analysis of fosmid clones and uncloned metagenomic DNA, revealed a diverse and abundant aminoglycoside and beta-lactam resistance reservoir within the infant gut, with resistance determinants exhibiting homology to those found in common gut inhabitants, including Escherichia coli, Enterococcus sp., and Clostridium difficile, as well as to genes from cryptic environmental bacteria. Notably, the genes identified differed from those revealed when a sequence-driven PCR-based screen of metagenomic DNA was employed. Carriage of these antibiotic resistance determinants conferred substantial, but varied (2-512x), increases in antibiotic resistance to their bacterial host. These data provide insights into the infant gut resistome, revealing the presence of a varied aminoglycoside and beta-lactam resistance reservoir even in the absence of selective pressure, confirming the infant resistome establishes early in life, perhaps even at birth. |
en |
dc.description.sponsorship |
Irish Research Council (EMBARK scholarship); Science Foundation Ireland (Investigator Award 11/PI/1137); Medical Research Council, United Kingdom (Grant No. G0901553) |
en |
dc.format.mimetype |
application/pdf |
en |
dc.language.iso |
en |
en |
dc.publisher |
Public Library of Science |
en |
dc.rights |
© 2015 Fouhy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Antibiotic resistance |
en |
dc.subject |
Intestinal microbiota |
en |
dc.subject |
Staphylococcus aureus |
en |
dc.subject |
Escherichia coli |
en |
dc.subject |
CTX-M |
en |
dc.subject |
Reveals |
en |
dc.subject |
PCR |
en |
dc.subject |
Enterococci |
en |
dc.subject |
Ampicillin |
en |
dc.subject |
Enzymes |
en |
dc.title |
Identification of aminoglycoside and beta-lactam resistance genes from within an infant gut functional metagenomic library |
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dc.type |
Article (peer-reviewed) |
en |
dc.internal.authorcontactother |
Paul Ross, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. +353-21-490-3000 Email: p.ross@ucc.ie |
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dc.internal.availability |
Full text available |
en |
dc.description.version |
Published Version |
en |
dc.internal.rssid |
275815832 |
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dc.internal.wokid |
WOS:000342351800052 |
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dc.contributor.funder |
Irish Research Council |
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dc.contributor.funder |
Teagasc |
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dc.contributor.funder |
Science Foundation Ireland |
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dc.contributor.funder |
Irish Government |
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dc.contributor.funder |
APC Microbiome Institute, College of Medicine and Health, University College Cork |
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dc.contributor.funder |
Medical Research Council, United Kingdom |
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dc.contributor.funder |
Society for Applied Microbiology, United Kingdom |
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dc.contributor.funder |
University of Brighton, United Kingdom |
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dc.contributor.funder |
Healthcare Infection Society, United Kingdom |
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dc.description.status |
Peer reviewed |
en |
dc.identifier.journaltitle |
PLOS ONE |
en |
dc.internal.IRISemailaddress |
g.dempsey@ucc.ie |
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dc.internal.IRISemailaddress |
p.ross@ucc.ie |
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dc.internal.IRISemailaddress |
p.ross@ucc.ie |
en |
dc.identifier.articleid |
e108016 |
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