A non-classical LysR-type transcriptional regulator PA2206 is required for an effective oxidative stress response in Pseudomonas aeruginosa

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dc.contributor.author Reen, F. Jerry
dc.contributor.author Haynes, Jill M.
dc.contributor.author Mooij, Marlies J.
dc.contributor.author O'Gara, Fergal
dc.date.accessioned 2016-02-17T11:46:20Z
dc.date.available 2016-02-17T11:46:20Z
dc.date.issued 2013
dc.identifier.citation Reen FJ, Haynes JM, Mooij MJ, O'Gara F (2013) A Non-Classical LysR-Type Transcriptional Regulator PA2206 Is Required for an Effective Oxidative Stress Response in Pseudomonas aeruginosa. PLoS ONE 8(1): e54479. doi:10.1371/journal.pone.0054479 en
dc.identifier.volume 8 en
dc.identifier.issued 1 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2395
dc.identifier.doi 10.1371/journal.pone.0054479
dc.description.abstract LysR-type transcriptional regulators (LTTRs) are emerging as key circuit components in regulating microbial stress responses and are implicated in modulating oxidative stress in the human opportunistic pathogen Pseudomonas aeruginosa. The oxidative stress response encapsulates several strategies to overcome the deleterious effects of reactive oxygen species. However, many of the regulatory components and associated molecular mechanisms underpinning this key adaptive response remain to be characterised. Comparative analysis of publically available transcriptomic datasets led to the identification of a novel LTTR, PA2206, whose expression was altered in response to a range of host signals in addition to oxidative stress. PA2206 was found to be required for tolerance to H2O2 in vitro and lethality in vivo in the Zebrafish embryo model of infection. Transcriptomic analysis in the presence of H2O2 showed that PA2206 altered the expression of 58 genes, including a large repertoire of oxidative stress and iron responsive genes, independent of the master regulator of oxidative stress, OxyR. Contrary to the classic mechanism of LysR regulation, PA2206 did not autoregulate its own expression and did not influence expression of adjacent or divergently transcribed genes. The PA2214-15 operon was identified as a direct target of PA2206 with truncated promoter fragments revealing binding to the 5'-ATTGCCTGGGGTTAT-3' LysR box adjacent to the predicted -35 region. PA2206 also interacted with the pvdS promoter suggesting a global dimension to the PA2206 regulon, and suggests PA2206 is an important regulatory component of P. aeruginosa adaptation during oxidative stress. en
dc.description.sponsorship European Commission (MTKD-CT-2006-042062, O36314, FP7-PEOPLE-2009-RG, EU 256596); Science Foundation Ireland (SFI 04/BR/B0597, 07/IN.1/B948, 08/RFP/GEN1295, 08/RFP/GEN1319, 09/RFP/BMT2350); Department of Agriculture and Food (DAF RSF 06 321, DAF RSF 06 377, FIRM 08/RDC/629); Irish Research Council for Science, Engineering and Technology (05/EDIV/FP107, PD/2011/2414); Health Research Board (RP/2006/271, RP/2007/290, HRA/2009/146); Environmental Protection Agency (EPA2006-PhD-S-21, EPA2008-PhD-S-2); Marine Institute (C2CRA 2007/082); Higher Education Authority of Ireland (PRTLI3); Health Service Executive (HSE) surveillance fund en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2013 Reen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Cystic fibrosis patients en
dc.subject Escherichia coli en
dc.subject Hydrogen peroxide en
dc.subject Salmonella typhimurium en
dc.subject Sinorhizobium meliloti en
dc.subject Opportunistic pathogen en
dc.subject Human neutrophils en
dc.subject Gene expression en
dc.subject Full virulence en
dc.subject OxyR en
dc.title A non-classical LysR-type transcriptional regulator PA2206 is required for an effective oxidative stress response in Pseudomonas aeruginosa en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother F. Jerry Reen, Microbiology University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.reen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 206307599
dc.internal.wokid WOS:000315211500013
dc.contributor.funder European Commission en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Department of Agriculture and Food, Ireland en
dc.contributor.funder Irish Research Council for Science Engineering and Technology en
dc.contributor.funder Health Research Board en
dc.contributor.funder Environmental Protection Agency en
dc.contributor.funder Marine Institute en
dc.contributor.funder Higher Education Authority en
dc.contributor.funder Health Service Executive, Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress f.ogara@ucc.ie en
dc.identifier.articleid e54479


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© 2013 Reen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2013 Reen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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