Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model
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Date
2013-06-20
Authors
Huang, Shungen
Li, Yi Ping
Zhang, Qi
Redmond, H. Paul
Wang, Jiang Huai
Wang, Jian
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central Ltd.
Published Version
Abstract
Background: Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible to microbial infection. Results: BALB/c mice were randomized into control, laparotomy, and laparoscopy groups. Laparotomy, but not laparoscopy, significantly downregulated CR3 expression on macrophages, diminished macrophage-induced uptake and phagocytosis of E. coli and S. aureus, and impaired macrophage-mediated intracellular bacterial killing. Consistent with this, mice that underwent laparotomy displayed substantially higher bacterial counts in the blood and visceral organs as well as a significantly enhanced mortality rate following bacterial infection, whereas mice subjected to laparoscopy did not show any defects in their bacterial clearance. Conclusion: Laparotomy has an adverse effect on host innate immunity against microbial infection by impairing macrophage-mediated phagocytosis and killing of the invaded bacteria. By contrast, laparoscopy appears to preserve macrophage-associated bactericidal ability, thus alleviating the development of postoperative immunosuppression.
Description
Keywords
Laparotomy , Laparoscopy , Phagocytosis , Bactericidal activity , Innate immunity , Macrophages
Citation
HUANG, S. G., LI, Y. P., ZHANG, Q., REDMOND, H. P., WANG, J. H. & WANG, J. 2013. Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model. BMC Immunology, 14:27, 1-8. http://dx.doi.org/10.1186/1471-2172-14-27