Neutral red retention time assay in determination of toxicity of nanoparticles

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dc.contributor.author Hu, Wentao
dc.contributor.author Culloty, Sarah C.
dc.contributor.author Darmody, Grainne
dc.contributor.author Lynch, Sharon A.
dc.contributor.author Davenport, John
dc.contributor.author Ramírez-García, Sonia
dc.contributor.author Dawson, Kenneth
dc.contributor.author Lynch, Iseult
dc.contributor.author Doyle, Hugh
dc.contributor.author Sheehan, David
dc.date.accessioned 2016-04-19T08:36:44Z
dc.date.available 2016-04-19T08:36:44Z
dc.date.issued 2015-05-16
dc.identifier.citation HU, W., CULLOTY, S., DARMODY, G., LYNCH, S., DAVENPORT, J., RAMIREZ-GARCIA, S., DAWSON, K., LYNCH, I., DOYLE, H. & SHEEHAN, D. 2015. Neutral red retention time assay in determination of toxicity of nanoparticles. Marine Environmental Research, 111, 158-161. doi:10.1016/j.marenvres.2015.05.007 en
dc.identifier.volume 111 en
dc.identifier.startpage 158 en
dc.identifier.endpage 161 en
dc.identifier.issn 0141-1136
dc.identifier.uri http://hdl.handle.net/10468/2456
dc.identifier.doi 10.1016/j.marenvres.2015.05.007
dc.description.abstract The neutral red retention time (NRRT) assay is useful for detecting decreased lysosomal membrane stability in haemocytes sampled from bivalves, a phenomenon often associated with exposure to environmental pollutants including nanomaterials. Bivalves are popular sentinel species in ecotoxicology and use of NRRT in study of species in the genus Mytilus is widespread in environmental monitoring. The NRRT assay has been used as an in vivo test for toxicity of carbon nanoparticles (Moore MN, Readman JAJ, Readman JW, Lowe DM, Frickers PE, Beesley A. 2009. Lysosomal cytotoxicity of carbon nanoparticles in cells of the molluscan immune system: An in vivo study. Nanotoxicology. 3 (1), 40-45). We here report application of this assay adapted to a microtitre plate format to a panel of metal and metal oxide nanoparticles (2 ppm). This showed that copper, chromium and cobalt nanoparticles are toxic by this criterion while gold and titanium nanoparticles are not. As the former three nanoparticles are often reported to be cytotoxic while the latter two are thought to be non-cytotoxic, these data support use of NRRT as a general in vitro assay in nanotoxicology. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier B.V. en
dc.relation.uri http://dx.doi.org/10.1016/j.marenvres.2015.05.007
dc.rights Copyright © 2015 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Mytilus en
dc.subject Metal oxide en
dc.subject Lysosome en
dc.subject Membrane stability en
dc.title Neutral red retention time assay in determination of toxicity of nanoparticles en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Hugh Doyle, Micro-Nanoelectronics Centre, Tyndall National Institute, Cork, Ireland. +353-21-234-6722 Email: hugh.doyle@tyndall.ie en
dc.internal.availability Full text not available en
dc.check.info Access to this article is restricted until 24 months after publication by the request of the publisher. en
dc.check.date 2017-05-16
dc.date.updated 2016-04-10T17:49:16Z
dc.description.version Accepted Version en
dc.internal.rssid 343806511
dc.description.status Peer reviewed en
dc.identifier.journaltitle Marine Environmental Research en
dc.internal.copyrightchecked Yes !!CORA!! AV permitted by publisher after 24 month embargo as referenced on Sherpa RoMEO and on publisher's website https://www.elsevier.com/about/company-information/policies/sharing en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress hugh.doyle@tyndall.ie en


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Copyright © 2015 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ Except where otherwise noted, this item's license is described as Copyright © 2015 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/
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