Analysis of expression quantitative trait loci in D. melanogaster

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dc.contributor.advisor Moore, Thomas F. en
dc.contributor.advisor Furlong, Eileen en
dc.contributor.author Harnett, Dermot
dc.date.accessioned 2016-09-23T10:06:46Z
dc.date.issued 2016
dc.date.submitted 2016
dc.identifier.citation Harnett, D. 2016. Analysis of expression quantitative trait loci in D. melanogaster. PhD Thesis, University College Cork. en
dc.identifier.endpage 204 en
dc.identifier.uri http://hdl.handle.net/10468/3114
dc.description.abstract Expression Quantitative Trait Loci (eQTL) analysis allows for the identification of genetic variation associated with variation in gene expression. It is often unclear however, which of the associated variants are causal, and by what mechanism. Integrating functional genomic data with eQTL data can provide insight into the impact of natural variation in the population, and the nature of the transcriptional machinery itself. In this thesis, I integrate functional genomic data with eQTL data derived from both 5’ CAGE and 3’ TagXseq expression assays, in developing embryos. I first use both datasets to analyse the transcription landscape in embryonic D., melanogaster, and then carry out an analysis of sequence motifs associated with transcription factor binding sites, promoters, and 3’ polyadenylation sites. Finally, I integrate functional genomic data, including these novel sequence motifs, to shed light on the mechanisms of gene expression variation in D.,melanogaster. I am able to demonstrate that some variants effecting gene regulation in Drosophila are found within haplotypes which buffer their effects. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2016, Dermot Harnett. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Genetics en
dc.subject Drosophila en
dc.title Analysis of expression quantitative trait loci in D. melanogaster en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en
dc.description.version Accepted Version
dc.description.status Not peer reviewed en
dc.internal.school Biochemistry en
dc.check.reason This thesis is due for publication or the author is actively seeking to publish this material en
dc.check.opt-out No en
dc.thesis.opt-out false
dc.check.entireThesis Entire Thesis Restricted
dc.check.embargoformat Both hard copy thesis and e-thesis en
ucc.workflow.supervisor t.moore@ucc.ie
dc.internal.conferring Autumn 2016 en


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© 2016, Dermot Harnett. Except where otherwise noted, this item's license is described as © 2016, Dermot Harnett.
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