Identification and characterization of a glycosulfatase-encoding gene cluster in Bifidobacterium breve UCC2003

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dc.contributor.author Egan, Muireann
dc.contributor.author Jiang, Hao
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Oscarson, Stefan
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2016-09-28T11:59:54Z
dc.date.available 2016-09-28T11:59:54Z
dc.date.issued 2016-09
dc.identifier.citation Egan, M., Jiang, H., O'Connell Motherway, M., Oscarson, S. and Van Sinderen, D. (2016) ‘Identification and characterization of a glycosulfatase-encoding gene cluster in Bifidobacterium breve UCC2003’, Applied and Environmental Microbiology, 82(22), pp. 6611-6623. doi: 10.1128/aem.02022-16 en
dc.identifier.volume 82
dc.identifier.issued 22
dc.identifier.startpage 6611
dc.identifier.endpage 6623
dc.identifier.issn 1098-5336
dc.identifier.uri http://hdl.handle.net/10468/3130
dc.identifier.doi 10.1128/AEM.02022-16
dc.description.abstract Bifidobacteria constitute a specific group of commensal bacteria, typically found in the gastrointestinal tract (GIT) of humans and other mammals. Bifidobacterium breve strains are numerically prevalent among the gut microbiota of many healthy breast-fed infants. In the current study, we investigated glycosulfatase activity in a bacterial nursling stool isolate, B. breve UCC2003. Two putative sulfatases were identified on the genome of B. breve UCC2003. The sulfated monosaccharide N-acetylglucosamine-6-sulfate (GlcNAc-6-S) was shown to support growth of B. breve UCC2003, while, N-acetylglucosamine-3-sulfate, N-acetylgalactosamine-3-sulfate and N-acetylgalactosamine-6-sulfate, did not support appreciable growth. Using a combination of transcriptomic and functional genomic approaches, a gene cluster, designated ats2, was shown to be specifically required for GlcNAc-6-S metabolism. Transcription of the ats2 cluster is regulated by a ROK-family transcriptional repressor. This study represents the first description of glycosulfatase activity within the Bifidobacterium genus. Bifidobacteria are saccharolytic organisms naturally found in the digestive tract of mammals and insects. Bifidobacterium breve strains utilize a variety of plant and host-derived carbohydrates which allow them to be present as prominent members of the infant gut microbiota as well as being present in the gastrointestinal tract of adults. In this study, we introduce a previously unexplored area of carbohydrate metabolism in bifidobacteria, namely the metabolism of sulfated carbohydrates. B. breve UCC2003 was shown to metabolize N-acetylglucosamine-6-sulfate (GlcNAc-6-S) through one of two sulfatase-encoding gene clusters identified on its genome. GlcNAc-6-S can be found in terminal or branched positions of mucin oligosaccharides, the glycoprotein component of the mucous layer that covers the digestive tract. The results of this study provide further evidence of this species' ability to utilize mucin-derived sugars, a trait which may provide a competitive advantage in both the infant and adult gut. en
dc.description.sponsorship Science Foundation Ireland (07/CE/B1368, SI/12/RC/2273, 13/IA/1959); Health Research Board (512 PDTM/20011/9) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.relation.uri http://aem.asm.org/content/early/2016/08/29/AEM.02022-16.abstract
dc.rights © 2016, American Society for Microbiology. All Rights Reserved. en
dc.subject Bifidobacterium breve en
dc.subject Carbohydrate metabolism en
dc.title Identification and characterization of a glycosulfatase-encoding gene cluster in Bifidobacterium breve UCC2003 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Muireann Egan, APC Microbiome Institute, University College Cork, Cork, Ireland. +353-21-490-3000 Email: muireann.egan@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 6 months after publication by the request of the publisher. en
dc.check.date 2017-03-02
dc.date.updated 2016-09-28T11:30:27Z
dc.description.version Accepted Version en
dc.internal.rssid 365845667
dc.internal.pmid 27590817
dc.contributor.funder Health Research Board en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Applied and Environmental Microbiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en


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