Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo

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dc.contributor.author O'Mahony, Conor
dc.contributor.author Houlihan, Ruth
dc.contributor.author Grygoryev, Konstantin
dc.contributor.author Ning, Zhenfei
dc.contributor.author Williams, John
dc.contributor.author Moore, Thomas F.
dc.date.accessioned 2016-11-28T15:21:37Z
dc.date.available 2016-11-28T15:21:37Z
dc.date.issued 2016-11
dc.identifier.citation O'Mahony, C., Houlihan, R., Grygoryev, K., Ning, Z., Williams, J. and Moore, T. (2016) 'Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo'. Journal of Physics: Conference Series, 757 :012040-1-012040-6. doi: 10.1088/1742-6596/757/1/012040 en
dc.identifier.volume 757 en
dc.identifier.startpage 012040-1 en
dc.identifier.endpage 012040-6 en
dc.identifier.issn 1742-6588,
dc.identifier.uri http://hdl.handle.net/10468/3315
dc.identifier.doi 10.1088/1742-6596/757/1/012040
dc.description.abstract We analysed the use of microneedle-based electrodes to enhance electroporation of mouse testis with DNA vectors for production of transgenic mice. Different microneedle formats were developed and tested, and we ultimately used electrodes based on arrays of 500 μm tall microneedles. In a series of experiments involving injection of a DNA vector expressing Green Fluorescent Protein (GFP) and electroporation using microneedle electrodes and a commercially available voltage supply, we compared the performance of flat and microneedle electrodes by measuring GFP expression at various timepoints after electroporation. Our main finding, supported by both experimental and simulated data, is that needles significantly enhanced electroporation of testis. en
dc.description.sponsorship Higher Education Authority (PRTLI5 Irish Transgenic Network infrastructure grant); Science Foundation Ireland (SFI National Access Programme (NAP 374)) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher IOP Publishing en
dc.rights © 2016, The Authors. Published under licence by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. en
dc.rights.uri https://creativecommons.org/licenses/by/3.0/ en
dc.subject Microneedle formats en
dc.subject Microneedle electrodes en
dc.subject GFP expression en
dc.subject Electroporation en
dc.title Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Conor O'Mahony, Tyndall Microsystems, University College Cork, Cork, Ireland. +353-21-490-3000 Email: conor.omahony@tyndall.ie en
dc.internal.availability Full text available en
dc.date.updated 2016-11-28T15:09:40Z
dc.description.version Published Version en
dc.internal.rssid 373618760
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Higher Education Authority en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Physics: Conference Series en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress conor.omahony@tyndall.ie en


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© 2016, The Authors. Published under licence by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Except where otherwise noted, this item's license is described as © 2016, The Authors. Published under licence by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
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