Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus

cb
Thumbnail Image
Files
3724.pdf(2.49 MB)
Published Version
3724-1.doc(217.5 KB)
Extracted File 1
Date
2017
Authors
Kiran, George Seghal
Jackson, Stephen A.
Priyadharsini, Sethu
Dobson, Alan D. W.
Selvin, Joseph
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Published Version
10.1038/s41598-017-08816-y
Research Projects
Organizational Units
Journal Issue
Abstract
Melanin is a dark brown ubiquitous photosynthetic pigment which have many varied and ever expanding applications in fabrication of radio-protective materials, food packaging, cosmetics and in medicine. In this study, melanin production in a Pseudomonas sp. which was isolated from the marine sponge Tetyrina citirna was optimized employing one-factor at a time experiments and characterized for chemical nature and stability. Following sonication nucleated nanomelanin (Nm) particles were formed and evaluated for antibacterial and antioxidant properties. Nanocomposite film was fabricated using combinations (% w/v) of polyhydroxy butyrate-nanomelanin (PHB: Nm) blended with 1% glycerol. The Nm was found to be spherical in shape with a diameter of 100-140 nm and showed strong antimicrobial activity against both Gram positive and Gram negative bacteria. The Nm-PHB nanocomposite film was homogeneous, smooth, without any cracks, and flexible. XRD and DSC data indicated that the film was crystalline in nature, and was thermostable up to 281.87 degrees C. This study represents the first report on the synthesis of Nm and fabrication of Nm-PHB nanocomposite film which show strong protective effect against multidrug resistant Staphyloccoccus aureus. Thus this Nm-PHB nanocomposite film may find utility as packaging material for food products by protecting the food products from oxidation and bacterial contamination.
Description
Keywords
Melanin pigment , Dopamine , Plasticizers , Sponges , Enzymes , Growth
Citation
Kiran, G. S., Jackson, S. A., Priyadharsini, S., Dobson, A. D. W. and Selvin, J. (2017) 'Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus', Scientific Reports, 7(1), 9167 (13pp). doi: 10.1038/s41598-017-08816-y
Link to publisher’s version
https://www.nature.com/articles/s41598-017-08816-y
URI
https://hdl.handle.net/10468/4807
Collections
Microbiology - Journal Articles
Environmental Research Institute - Journal Articles
Copyright
© 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.