Another brick in the wall: a rhamnan polysaccharide trapped inside peptidoglycan of Lactococcus lactis

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dc.contributor.author Sadovskaya, Irina
dc.contributor.author Vinogradov, Evgeny
dc.contributor.author Courtin, Pascal
dc.contributor.author Armalyte, Julija
dc.contributor.author Meyrand, Mickael
dc.contributor.author Giaouris, Efstathios
dc.contributor.author Palussière, Simon
dc.contributor.author Furlan, Sylviane
dc.contributor.author Péchoux, Christine
dc.contributor.author Ainsworth, Stuart
dc.contributor.author Mahony, Jennifer
dc.contributor.author van Sinderen, Douwe
dc.contributor.author Kulakauskas, Saulius
dc.contributor.author Guérardel, Yann
dc.contributor.author Chapot-Chartier, Marie-Pierre
dc.date.accessioned 2017-10-18T09:40:15Z
dc.date.available 2017-10-18T09:40:15Z
dc.date.issued 2017
dc.identifier.citation Sadovskaya, I., Vinogradov, E., Courtin, P., Armalyte, J., Meyrand, M., Giaouris, E., Palussière, S., Furlan, S., Péchoux, C., Ainsworth, S., Mahony, J., van Sinderen, D., Kulakauskas, S., Guérardel, Y. and Chapot-Chartier, M.-P. (2017) 'Another brick in the wall: a rhamnan polysaccharide trapped inside peptidoglycan of Lactococcus lactis', mBio, 8(5), e01303-17 (16pp). doi: 10.1128/mBio.01303-17 en
dc.identifier.volume 8
dc.identifier.issued 5
dc.identifier.startpage 1
dc.identifier.endpage 16
dc.identifier.issn 2150-7511
dc.identifier.uri http://hdl.handle.net/10468/4901
dc.identifier.doi 10.1128/mBio.01303-17
dc.description.abstract Polysaccharides are ubiquitous components of the Gram-positive bacterial cell wall. In Lactococcus lactis, a polysaccharide pellicle (PSP) forms a layer at the cell surface. The PSP structure varies among lactococcal strains; in L. lactis MG1363, the PSP is composed of repeating hexasaccharide phosphate units. Here, we report the presence of an additional neutral polysaccharide in L. lactis MG1363 that is a rhamnan composed of α-L-Rha trisaccharide repeating units. This rhamnan is still present in mutants devoid of the PSP, indicating that its synthesis can occur independently of PSP synthesis. High-resolution magic-angle spinning nuclear magnetic resonance (HR-MAS NMR) analysis of whole bacterial cells identified a PSP at the surface of wild-type cells. In contrast, rhamnan was detected only at the surface of PSP-negative mutant cells, indicating that rhamnan is located underneath the surface-exposed PSP and is trapped inside peptidoglycan. The genetic determinants of rhamnan biosynthesis appear to be within the same genetic locus that encodes the PSP biosynthetic machinery, except the gene tagO encoding the initiating glycosyltransferase. We present a model of rhamnan biosynthesis based on an ABC transporter-dependent pathway. Conditional mutants producing reduced amounts of rhamnan exhibit strong morphological defects and impaired division, indicating that rhamnan is essential for normal growth and division. Finally, a mutation leading to reduced expression of lcpA, encoding a protein of the LytR-CpsA-Psr (LCP) family, was shown to severely affect cell wall structure. In lcpA mutant cells, in contrast to wild-type cells, rhamnan was detected by HR-MAS NMR, suggesting that LcpA participates in the attachment of rhamnan to peptidoglycan. en
dc.description.sponsorship Agence Nationale de la Recherche (“Lactophages” (ANR-11-BSV8-004-01) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.uri http://mbio.asm.org/content/8/5/e01303-17
dc.rights © 2017, Sadovskaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject HR-MAS NMR en
dc.subject Lactococcus en
dc.subject Cell wall en
dc.subject Polysaccharides en
dc.subject Rhamnan en
dc.title Another brick in the wall: a rhamnan polysaccharide trapped inside peptidoglycan of Lactococcus lactis en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Jennifer Mahony, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.mahony@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Agence Nationale de la Recherche
dc.contributor.funder Conseil Régional, Île-de-France
dc.contributor.funder Institut National de la Recherche Agronomique
dc.contributor.funder Science Foundation Ireland
dc.description.status Peer reviewed en
dc.identifier.journaltitle mBio en
dc.internal.IRISemailaddress j.mahony@ucc.ie en
dc.identifier.articleid e01303-17
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/14/TIDA/2287/IE/Next generation diagnostic tools for problematic dairy bacteriophages/
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/


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© 2017, Sadovskaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Except where otherwise noted, this item's license is described as © 2017, Sadovskaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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