Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2

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Date
2017
Authors
Aprea, Giuseppe
Mullan, William Michael
Murru, Nicoletta
Fitzgerald, Gerald F.
Buonanno, Marialuisa
Cortesi, Maria Luisa
Prencipe, Vincenza Annunziata
Migliorat, Giacomo
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
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Abstract
This work investigated bacteriophage induced starter failures in artisanal buffalo Mozzarella production plants in Southern Italy. Two hundred and ten samples of whey starter cultures were screened for bacteriophage infection. Multiplex polymerase chain reaction (PCR) revealed phage infection in 28.56% of samples, all showing acidification problems during cheese making. Based on DNA sequences, bacteriophages for Lactococcus lactis (L. lactis), Lactobacillus delbruekii (L. delbruekii) and Streptococcus thermophilus (S. thermophilus) were detected. Two phages active against L.  lactis, фApr-1 and фApr-2, were isolated and characterised. The genomes, approximately 31.4 kb and 31 kb for фApr-1 and фApr-2 respectively, consisted of double-stranded linear DNA with pac-type system. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed one major structural protein of approximately 32.5 kDa and several minor proteins. This is the first report of phage isolation in buffalo milk and of the use of multiplex PCR to screen and study the diversity of phages against Lactic Acid Bacteria (LAB) strains in artisanal Water Buffalo Mozzarella starters.
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Bacteriophage , Buffalo , Lactic Acid Bacteria (LAB) , Lactococcus lactis , Milk , Natural starter culture , PCR
Citation
Aprea, G., Mullan, W. M., Murru, N., Fitzgerald, G., Buonanno, M., Cortesi, M. L., Prencipe, V. A. and Migliorati, G. (2017) 'Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2', Veterinaria Italiana, 53(3), pp. 207-214. doi: 10.12834/VetIt.315.1238.3
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© 2017, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise. Open Access:This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.