In vitro cytotoxicity of water soluble silver (Ag) nanoparticles on HaCat and A549 cell lines
Padmanabhan, Sibu C.
Pillai, Suresh C.
Cruz-Romero, Malco C.
Kerry, Joseph P.
Morris, Michael A.
Scientific Open Access Journals
The wide range of applications of silver nanoparticles (AgNPs) in commercial products, including food packaging, has encouraged researchers to come up with novel preparation methods for the production of these robust materials. The methods resulting in the formation of NPs for such commercial applications clearly demand a good accounting of their toxicity aspects to humans as well as the environment. We herein present a chemical preparation method for the production of size- and shape-defined AgNPs and investigate the impact of these nanoparticles on HaCat and A549 cell lines. Findings show that lung cells (A549) are more sensitive than skin cells (HaCat) to Ag induced toxicity, evident by the significantly (p<0.05) reduced LC50 for all NPs under study. The current investigation showed that the extent of surface capping agent (citrate) and size influenced the cell toxicity, where a lesser surface coverage (zeta potential, ζ, -27.7 mV) and smaller size (~17 nm) enhanced the toxicity compared to comparatively bigger particles (~39 nm) with higher surface coverage (ζ, -47.3 mV). The size- and shape-defined particles such as triangles which have proven useful for many applications, due to their high energy/high field edges, were found to be less toxic against biological cell lines and therefore may have potential to be used in food packaging applications as reservoirs of silver ions. A striking difference in cell line toxicity within such a small size window clearly demonstrates the vital roles played by the smaller size, difference in shape and lesser surface coverage in defining a higher passive cell membrane diffusion followed by silver dissolution inside cell cytoplasm increasing cytotoxicity.
Silver nanoparticles , Cytotoxicity , Lung cell , Skin cells
Garvey, M., Padmanabhan, S. and Pillai, S. (2017) 'In vitro cytotoxicity of water soluble silver (Ag) nanoparticles on HaCat and A549 cell lines', Journal of Toxicology and Pharmacology, 1(3), 016 (8pp)
© 2017, the Authors. Published by Scientific Open Access Journals LLC. Works published by the Scientific Open Access Journals are under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited; the original copy writes are retained to the author.