Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes

Show simple item record Dalfen, Irene Dmitriev, Ruslan I. Holst, Gerhard Klimant, Ingo Borisov, Sergey M. 2018-12-18T11:14:29Z 2018-12-18T11:14:29Z 2018-12-06
dc.identifier.citation Dalfen, I., Dmitriev, R. I., Holst, G., Klimant, I.and Borisov, S. M. (2018) 'Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes', Analytical Chemistry. doi:10.1021/acs.analchem.8b02534 en
dc.identifier.issn 0003-2700
dc.identifier.issn 1520-6882
dc.identifier.doi 10.1021/acs.analchem.8b02534
dc.description.abstract Novel fluorescent diazaoxatriangulenium (DAOTA) pH indicators for lifetime-based self-referenced pH sensing are reported. The DAOTA dyes were decorated with phenolic receptor groups inducing fluorescence quenching via photoinduced electron transfer mechanism. Electron-withdrawing chlorine substituents ensure response in the most relevant pH range (apparent pK'a values ~5 and 7.5 for the p,p-dichlorophenol- and the p-chlorophenol-substituted dyes, respectively). The dyes feature long fluorescence lifetime (17-20 ns), high quantum yield (~60%) and high photostability. Planar optodes are prepared upon immobilization of the dyes into polyurethane hydrogel D4. Apart from the response in the fluorescence intensity, the optodes show pH-dependent lifetime behaviour which makes them suitable for studying 2D pH distribution with help of fluorescence lifetime imaging technique. The lifetime response is particularly pronounced for the sensors with high dye concentration (0.5-1% wt. in respect to the polymer) and is attributed to efficient homo-FRET mechanism. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Chemical Society en
dc.rights © 2018, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry after technical editing by the publisher. To access the final edited and published work see en
dc.subject Decay time en
dc.subject FRET en
dc.subject pH sensor en
dc.subject Triangulenium en
dc.subject Photoinduced electron transfer en
dc.subject Frequency Domain FLIM en
dc.title Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Ruslan Dmitriev, Biochemistry , University College Cork, Cork, Ireland. +353-21-490-3000 Email: en
dc.internal.availability Full text available en Access to this article is restricted until 12 months after publication by request of the publisher. en 2019-12-06 2018-12-13T12:42:59Z
dc.description.version Accepted Version en
dc.internal.rssid 465418112
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Analytical Chemistry en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress en
dc.internal.bibliocheck In press. Check for vol. / issue / page numbers. Amend citation as necessary.
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/13/SIRG/2144/IE/Development of Bionic Sensor Materials for Metabolic Imaging in Regenerative Medicine/ en

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