Biochemical and biomolecular effects induced by a static magnetic field in Saccharomyces cerevisiae: evidence for oxidative stress
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Published version
Date
2019-01-04
Authors
Kthiri, Amena
Hidouri, Slah
Wiem, Tahri
Jeridi, Roua
Sheehan, David
Landouls, Ahmed
Journal Title
Journal ISSN
Volume Title
Publisher
PLoS ONE
Published Version
Abstract
Exposure to static magnetic fields (SMF) can cause changes in microorganism metabolism altering key subcellular functions. The purpose of this study was to investigate whether an applied SMF could induce biological effects on growth of Saccharomyces cerevisiae, and then to probe biochemical and bio-molecular responses. We found a decrease in growth and viability under SMF (250mT) after 6h with a significant decrease in colony forming units followed by an increase between 6 h and 9 h. Moreover, measurements of antioxidant enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) demonstrated a particular profile suggesting oxidative stress. For instance, SOD and catalase activities increased in magnetized cultures after 9 h compared with unexposed samples. However, SMF exposure caused a decrease in glutathione peroxidase activity. Finally, SMF caused an increase in MDA levels as well as the content of protein carbonyl groups after 6 and 9 h of exposure
Description
Keywords
Microorganism metabolism , Subcellular function , Static magnetic field
Citation
Kthiri, A., Hidouri, S., Wiem, T., Jeridi, R., Sheehan, D. and Landouls, A., 2019. Biochemical and biomolecular effects induced by a static magnetic field in Saccharomyces cerevisiae: Evidence for oxidative stress. PloS one, 14(1), e0209843, (12pp.). DOI: 10.1371/journal.pone.0209843