Implementation of transposon mutagenesis in Bifidobacterium

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dc.contributor.author Ruiz, Lorena
dc.contributor.author van Sinderen, Douwe
dc.contributor.editor Ricke, Steven C.
dc.contributor.editor Park, Si Hong
dc.contributor.editor Davis, Morgan L.
dc.date.accessioned 2019-07-08T12:05:00Z
dc.date.available 2019-07-08T12:05:00Z
dc.date.issued 2019-06-14
dc.identifier.citation Ruiz, L. and van Sinderen, D. (2019) 'Implementation of transposon mutagenesis in Bifidobacterium', in Ricke, S. C., Park, S. H. and Davis, M. L. (eds.) Microbial Transposon Mutagenesis: Protocols and Applications. Methods in Molecular Biology, vol. 2016. New York, Humana, pp. 51-62. doi: 10.1007/978-1-4939-9570-7_5 en
dc.identifier.volume 2016 en
dc.identifier.startpage 51 en
dc.identifier.endpage 62 en
dc.identifier.isbn 978-1-4939-9570-7
dc.identifier.uri http://hdl.handle.net/10468/8122
dc.identifier.doi 10.1007/978-1-4939-9570-7_5 en
dc.description.abstract Random transposon mutagenesis allows for relatively rapid, genome-wide surveys to detect genes involved in functional traits, by performing screens of mutant libraries. This approach has been widely applied to identify genes responsible for activities of interest in multiple eukaryote and prokaryote organisms, although most studies on microorganisms have focused on pathogenic and clinically relevant bacteria. In this chapter we describe the implementation of an in vitro Tn5-based transposome strategy to generate a large collection of random mutants in the gut commensal Bifidobacterium breve UCC2003, and discuss considerations when applying this mutagenesis system to other Bifidobacterium species or strains of interest. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Humana, New York, NY en
dc.relation.ispartof Microbial Transposon Mutagenesis: Protocols and Applications
dc.relation.ispartof Methods in Molecular Biology book series (MIMB, volume 2016)
dc.rights © 2019, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in Ricke, S. C., Park, S. H. and Davis, M. L. (eds.) Microbial Transposon Mutagenesis: Protocols and Applications. Methods in Molecular Biology, vol. 2016. New York, Humana, pp. 51-62. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9570-7_5 en
dc.subject Tn5 en
dc.subject Transposon en
dc.subject Bifidobacterium en
dc.title Implementation of transposon mutagenesis in Bifidobacterium en
dc.type Book chapter en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2020-06-14
dc.date.updated 2019-07-08T11:45:30Z
dc.description.version Accepted Version en
dc.internal.rssid 492044092
dc.description.status Not peer reviewed en
dc.identifier.journaltitle Methods in Molecular Biology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.placepublication New York en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en


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