Implementation of transposon mutagenesis in Bifidobacterium
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Date
14/06/2019
Authors
Ruiz, Lorena
van Sinderen, Douwe
Journal Title
Journal ISSN
Volume Title
Publisher
Humana, New York, NY
Published Version
Abstract
Random transposon mutagenesis allows for relatively rapid, genome-wide surveys to detect genes involved in functional traits, by performing screens of mutant libraries. This approach has been widely applied to identify genes responsible for activities of interest in multiple eukaryote and prokaryote organisms, although most studies on microorganisms have focused on pathogenic and clinically relevant bacteria. In this chapter we describe the implementation of an in vitro Tn5-based transposome strategy to generate a large collection of random mutants in the gut commensal Bifidobacterium breve UCC2003, and discuss considerations when applying this mutagenesis system to other Bifidobacterium species or strains of interest.
Description
Keywords
Tn5 , Transposon , Bifidobacterium
Citation
Ruiz, L. and van Sinderen, D. (2019) 'Implementation of transposon mutagenesis in Bifidobacterium', in Ricke, S. C., Park, S. H. and Davis, M. L. (eds.) Microbial Transposon Mutagenesis: Protocols and Applications. Methods in Molecular Biology, vol. 2016. New York, Humana, pp. 51-62. doi: 10.1007/978-1-4939-9570-7_5
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Copyright
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in Ricke, S. C., Park, S. H. and Davis, M. L. (eds.) Microbial Transposon Mutagenesis: Protocols and Applications. Methods in Molecular Biology, vol. 2016. New York, Humana, pp. 51-62. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9570-7_5