Host specific diversity in Lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms

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dc.contributor.author Guinane, Caitriona M.
dc.contributor.author Kent, Robert M.
dc.contributor.author Norberg, Sarah
dc.contributor.author Hill, Colin
dc.contributor.author Fitzgerald, Gerald F.
dc.contributor.author Stanton, Catherine
dc.contributor.author Ross, R. Paul
dc.contributor.editor Gagneux, Sebastien
dc.date.accessioned 2012-11-29T12:02:17Z
dc.date.available 2012-11-29T12:02:17Z
dc.date.copyright 2011
dc.date.issued 2011-04-20
dc.identifier.citation Guinane CM, Kent RM, Norberg S, Hill C, Fitzgerald GF, Stanton C, Ross RP. Host specific diversity in Lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms. PLoS One. 2011; 6(4):e18740. http://dx.doi.org/10.1371%2Fjournal.pone.0018740 en
dc.identifier.volume 6 en
dc.identifier.issued 4 en
dc.identifier.startpage e18740 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/823
dc.identifier.doi 10.1371/journal.pone.0018740
dc.description.abstract Genetic diversity and genomic rearrangements are a driving force in bacterial evolution and niche adaptation. We sequenced and annotated the genome of Lactobacillus johnsonii DPC6026, a strain isolated from the porcine intestinal tract. Although the genome of DPC6026 is similar in size (1.97mbp) and GC content (34.8%) to the sequenced human isolate L. johnsonii NCC 533, a large symmetrical inversion of approximately 750 kb differentiated the two strains. Comparative analysis among 12 other strains of L. johnsonii including 8 porcine, 3 human and 1 poultry isolate indicated that the genome architecture found in DPC6026 is more common within the species than that of NCC 533. Furthermore a number of unique features were annotated in DPC6026, some of which are likely to have been acquired by horizontal gene transfer (HGT) and contribute to protection against phage infection. A putative type III restriction-modification system was identified, as were novel Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) elements. Interestingly, these particular elements are not widely distributed among L. johnsonii strains. Taken together these data suggest intra-species genomic rearrangements and significant genetic diversity within the L. johnsonii species and indicate towards a host-specific divergence of L. johnsonii strains with respect to genome inversion and phage exposure. en
dc.description.sponsorship Science Foundation Ireland (SFI-CSET); Enterprise Ireland (CFTD/07/11) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLOS en
dc.relation.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018740
dc.rights 2011 Guinane et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ en
dc.subject Lactobacillus johnsonii en
dc.subject Horizontal gene transfer en
dc.subject Phage en
dc.subject Clustered regularly Interspaced Short Palindromic Repeats en
dc.subject Chromosomal inversion en
dc.subject Genomic rearrangement en
dc.title Host specific diversity in Lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://publish.ucc.ie/researchprofiles/D010/chill en
dc.internal.authorcontactother Colin Hill, Department of Microbiology, University College, Cork, Ireland. Email: c.hill@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 121848267
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Enterprise Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress c.hill@ucc.ie en


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2011 Guinane et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as 2011 Guinane et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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