Using high-density mutagenesis to identify the genetic requirements for the growth of Escherichia coli

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dc.contributor.advisor Clarke, David J. en Buttimer, Finbarr James 2019-08-22T10:22:21Z 2019 2019
dc.identifier.citation Buttimer, F. J. 2019. Using high-density mutagenesis to identify the genetic requirements for the growth of Escherichia coli. PhD Thesis, University College Cork. en
dc.identifier.endpage 377 en
dc.description.abstract Escherichia coli is highly adapted to life within the mammalian gastrointestinal (GI) tract, capable of adapting to multiple environments en route to colonising the intestine. Moreover, the means by which the species copes with changes in the microenvironments of the GI tract strongly influences the nature of E. coli's relationship with the host i.e. whether it exists as a commensal or pathogen. However, the response of E. coli to many of these conditions is complex, often employing a whole-cell response. This necessitates the use of high-throughput approaches in order to fully understand factors the bacterium requires for growth under these conditions. This work outlines the use of transposon-directed insertion site sequencing (TraDIS) to describe genetic requirements for fitness of E. coli K-12 MG1655 during growth in the presence of bile and under anaerobic conditions in the presence of nitrate, representative of bile exposure in the duodenum and conditions of inflammation in the intestine. TraDIS reveals, in detail, genetic requirements for growth under these conditions, revealing new roles for many genes with no prior association with growth under these conditions. This work will, therefore, contribute to future studies of E. coli colonisation of the GI tract by identifying candidate genes required for fitness under these growth conditions. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2019, Finbarr James Buttimer. en
dc.rights.uri en
dc.subject E. coli en
dc.subject TraDIS en
dc.subject Bile en
dc.subject Nitrate en
dc.title Using high-density mutagenesis to identify the genetic requirements for the growth of Escherichia coli en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD en
dc.internal.availability Full text not available en Restricted to everyone for three years en 2022-08-21T10:22:21Z
dc.description.version Accepted Version
dc.contributor.funder Irish Research Council en
dc.description.status Not peer reviewed en Microbiology en
dc.check.reason This thesis is due for publication or the author is actively seeking to publish this material en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.entireThesis Entire Thesis Restricted
dc.check.embargoformat Apply the embargo to the e-thesis on CORA (If you have submitted an e-thesis and want to embargo it on CORA) en
dc.internal.conferring Autumn 2019 en
dc.internal.ricu APC Microbiome Institute en
dc.relation.project Irish Research Council (GOIPG/2014/686) en

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© 2019, Finbarr James Buttimer. Except where otherwise noted, this item's license is described as © 2019, Finbarr James Buttimer.
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