Redox proteomic insights into involvement of clathrin-mediated endocytosis in silver nanoparticles toxicity to Mytilus galloprovincialis
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Date
2018-10-29
Authors
Bouallegui, Younes
Ben Younes, Ridha
Oueslati, Ridha
Sheehan, David
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Journal ISSN
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Publisher
PLOS
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Abstract
Clathrin-mediated endocytosis is a major mode of nanoparticle (NP) internalization into cells. However, influence of internalization routes on nanoparticle toxicity is poorly understood. Here, we assess the impact of blocking clathrin-mediated endocytosis upon silver NP (AgNP) toxicity to gills and digestive glands of the mussel Mytilusgalloprovincialisusing the uptake inhibitor, amantadine. Animals were exposed for 12h to AgNP (< 50 nm) in the presence and absence of amantadine. Labeling of oxidative protein modifications, either thiol oxidation, carbonyl formation or both in two-dimensional electrophoresis separations revealed 16 differentially affected abundance spots. Amongst these, twelve hypothetical proteins were successfully identified by peptide mass fingerprinting (MALDI TOF-MS/MS). The proteins identified are involved in buffering redox status or in cytoprotection. We conclude that blockade of clathrin-mediated endocytosis protected against NP toxicity, suggesting this uptake pathway facilitates toxicity. Lysosomal degradation and autophagy are major mechanisms that might be induced to mitigate NP toxicity.
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Keywords
Clathrin-mediated endocytosis , Nanoparticle (NP) , Mytilusgalloprovincialisusing , Lysosomal degradation , Autophagy
Citation
Bouallegui, Y., Younes, R.B., Oueslati, R. and Sheehan, D., 2018. Redox proteomic insights into involvement of clathrin-mediated endocytosis in silver nanoparticles toxicity to Mytilus galloprovincialis. PloS one, 13(10), (e0205765). DOI:10.1371/journal.pone.0205765