Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts

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dc.contributor.author Payelleville, Amaury
dc.contributor.author Blackburn, Dana
dc.contributor.author Lanois, Anne
dc.contributor.author Pagès, Sylvie
dc.contributor.author Cambon, Marine C.
dc.contributor.author Ginibre, Nadege
dc.contributor.author Clarke, David J.
dc.contributor.author Givaudan, Alain
dc.contributor.author Brillard, Julien
dc.date.accessioned 2019-10-26T06:49:47Z
dc.date.available 2019-10-26T06:49:47Z
dc.date.issued 2019-10-09
dc.identifier.citation Payelleville, A., Blackburn, D., Lanois, A., Pagès, S., Cambon, M. C., Ginibre, N., Clarke, D. J., Givaudan, A. and Brillard, J. (2019) 'Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts', PLOS ONE, 14(10), e0212655. (14pp.) DOI: 10.1371/journal.pone.0212655 en
dc.identifier.volume 14 en
dc.identifier.issued 10 en
dc.identifier.startpage 1 en
dc.identifier.endpage 14 en
dc.identifier.uri http://hdl.handle.net/10468/8872
dc.identifier.doi 10.1371/journal.pone.0212655 en
dc.description.abstract Photorhabdus luminescens is an entomopathogenic bacterium found in symbiosis with the nematode Heterorhabditis. Dam DNA methylation is involved in the pathogenicity of many bacteria, including P. luminescens, whereas studies about the role of bacterial DNA methylation during symbiosis are scarce. The aim of this study was to determine the role of Dam DNA methylation in P. luminescens during the whole bacterial life cycle including during symbiosis with H. bacteriophora. We constructed a strain overexpressing dam by inserting an additional copy of the dam gene under the control of a constitutive promoter in the chromosome of P. luminescens and then achieved association between this recombinant strain and nematodes. The dam overexpressing strain was able to feed the nematode in vitro and in vivo similarly as a control strain, and to re-associate with Infective Juvenile (IJ) stages in the insect. No difference in the amount of emerging IJs from the cadaver was observed between the two strains. Compared to the nematode in symbiosis with the control strain, a significant increase in LT50 was observed during insect infestation with the nematode associated with the dam overexpressing strain. These results suggest that during the life cycle of P. luminescens, Dam is not involved the bacterial symbiosis with the nematode H. bacteriophora, but it contributes to the pathogenicity of the nemato-bacterial complex. en
dc.description.sponsorship GAIA doctoral school (#584);Académie d’Agriculture de France (Dufrenoy Grant); INRA Plant Health and Environment (Society of Petroleum Engineers, SPE-IB17-DiscriMet); French ministries MEAE and MESRI (PHC ULYSSES 2018 grant); French National Research Agency (EPI-PATH project, ANR-17-CE20-0005) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.relation.uri https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212655
dc.rights ©2019 Payelleville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Symbiosis en
dc.subject DNA methylation en
dc.subject Pathogenesis en
dc.subject Bacterial pathogens en
dc.subject Insects en
dc.subject Larvae en
dc.subject Nematode infections en
dc.subject Plasmid construction en
dc.title Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Dana Blackburn, Department of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email:dana.blackburn@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Ministère de l'Enseignement supérieur, de la Recherche et de l'Innovation en
dc.contributor.funder Agence Nationale de la Recherche en
dc.contributor.funder Society of Petroleum Engineers en
dc.contributor.funder Académie d’Agriculture de France en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress dana.blackburn@ucc.ie en
dc.identifier.articleid e0212655 en
dc.identifier.eissn 1932-6203


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©2019 Payelleville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as ©2019 Payelleville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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