Microneedle array design determines the induction of protective memory CD8+ T Cell responses induced by a recombinant live malaria vaccine in mice

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dc.contributor.author Carey, John B.
dc.contributor.author Pearson, Frances E.
dc.contributor.author Vrdoljak, Anto
dc.contributor.author McGrath, Marie G.
dc.contributor.author Crean, Abina M.
dc.contributor.author Walsh, Patrick T.
dc.contributor.author Doody, Timothy
dc.contributor.author O'Mahony, Conor
dc.contributor.author Hill, Adrian V. S.
dc.contributor.author Moore, Anne C.
dc.date.accessioned 2019-11-19T10:19:45Z
dc.date.available 2019-11-19T10:19:45Z
dc.date.issued 2011-07-25
dc.identifier.citation Carey, J.B., Pearson, F.E., Vrdoljak, A., McGrath, M.G., Crean, A.M., Walsh, P.T., Doody, T., O'Mahony, C., Hill, A.V. and Moore, A.C., 2011. Microneedle array design determines the induction of protective memory CD8+ T cell responses induced by a recombinant live malaria vaccine in mice. PLoS One, 6(7), (e22442). DOI:10.1371/journal.pone.0022442 en
dc.identifier.volume 6 en
dc.identifier.issued 7 en
dc.identifier.startpage 1 en
dc.identifier.endpage 13 en
dc.identifier.uri http://hdl.handle.net/10468/9063
dc.identifier.doi 10.1371/journal.pone.0022442 en
dc.description.abstract Background: Vaccine delivery into the skin has received renewed interest due to ease of access to the immune system and microvasculature, however the stratum corneum (SC), must be breached for successful vaccination. This has been achieved by removing the SC by abrasion or scarification or by delivering the vaccine intradermally (ID) with traditional needle-and-syringes or with long microneedle devices. Microneedle patch-based transdermal vaccine studies have predominantly focused on antibody induction by inactivated or subunit vaccines. Here, our principal aim is to determine if the design of a microneedle patch affects the CD8+ T cell responses to a malaria antigen induced by a live vaccine. Methodology and Findings Recombinant modified vaccinia virus Ankara (MVA) expressing a malaria antigen was percutaneously administered to mice using a range of silicon microneedle patches, termed ImmuPatch, that differed in microneedle height, density, patch area and total pore volume. We demonstrate that microneedle arrays that have small total pore volumes induce a significantly greater proportion of central memory T cells that vigorously expand to secondary immunization. Microneedle-mediated vaccine priming induced significantly greater T cell immunity post-boost and equivalent protection against malaria challenge compared to ID vaccination. Notably, unlike ID administration, ImmuPatch-mediated vaccination did not induce inflammatory responses at the site of immunization or in draining lymph nodes. Conclusions/Significance: This study demonstrates that the design of microneedle patches significantly influences the magnitude and memory of vaccine-induced CD8+ T cell responses and can be optimised for the induction of desired immune responses. Furthermore, ImmuPatch-mediated delivery may be of benefit to reducing unwanted vaccine reactogenicity. In addition to the advantages of low cost and lack of pain, the development of optimised microneedle array designs for the induction of T cell responses by live vaccines aids the development of solutions to current obstacles of immunization programmes. en
dc.description.sponsorship CFTD 07/117; NAP156; NAP170; G0600311 en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLoS en
dc.relation.uri https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0022442
dc.rights © 2011 Carey et al en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Vaccine delivery en
dc.subject Microneedle en
dc.subject CD8+ T cell responses en
dc.title Microneedle array design determines the induction of protective memory CD8+ T Cell responses induced by a recombinant live malaria vaccine in mice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Anne Moore, School of Biochemistry and Cell biology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: anne.moore@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Enterprise Ireland en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Medical Research Council, United Kingdom en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.IRISemailaddress anne.moore@ucc.ie en
dc.internal.IRISemailaddress conor.omahony@tyndall.ie en
dc.identifier.articleid e22442 en
dc.identifier.eissn 1932-6203

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© 2011 Carey et al Except where otherwise noted, this item's license is described as © 2011 Carey et al
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