Comparative genetic and physiological characterisation of Pectinatus species reveals shared tolerance to beer-associated stressors but halotolerance specific to pickle-associated strains

Loading...
Thumbnail Image
Files
12212.pdf(862.27 KB)
Accepted version
Date
2020-02-21
Authors
Kramer, Timo
Kelleher, Philip
van der Meer, Julia
O'Sullivan, Tadhg
Geertman, Jan-Maarten A.
Duncan, Sylvia H.
Flint, Harry J.
Louis, Petra
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Research Projects
Organizational Units
Journal Issue
Abstract
Obligate anaerobic bacteria from the genus Pectinatus have been known to cause beer spoilage for over 40 years. Whole genome sequencing was performed on eleven beer spoilage strains (nine Pectinatus frisingensis, one Pectinatus cerevisiiphilus and one Pectinatus haikarae isolate), as well as two pickle spoilage species (Pectinatus brassicae MB591 and Pectinatus sottacetonis MB620) and the tolerance of all species to a range of environmental conditions was tested. Exploration of metabolic pathways for carbohydrates, amino acids and vitamins showed little difference between beer spoilage- and pickle spoilage-associated strains. However, genes for certain carbohydrate- and sulphur-containing amino acid-associated enzymes were only present in the beer spoilage group and genes for specific transporters and regulatory genes were uniquely found in the pickle spoilage group. Transporters for compatible solutes, only present in pickle-associated strains, likely explain their experimentally observed higher halotolerance compared to the beer spoilers. Genes involved in biofilm formation and ATP Binding Cassette (ABC) transporters potentially capable of exporting hop-derived antimicrobial compounds were found in all strains. All species grew in the presence of alcohol up to 5% alcohol by volume (ABV) and hops extract up to 80 ppm of iso-α-acids. Therefore, the species isolated from pickle processes may pose novel hazards in brewing.
Description
Keywords
Pectinatus , Comparative genomics , Environmental adaptation , Beer , Pickle , Spoilage
Citation
Kramer, T., Kelleher, P., van der Meer, J., O'Sullivan, T., Geertman, J.-M. A., Duncan, S. H., Flint, H. J. and Louis, P. (2020) 'Comparative genetic and physiological characterisation of Pectinatus species reveals shared tolerance to beer-associated stressors but halotolerance specific to pickle-associated strains', Food Microbiology, 90, 103462 (12 pp). doi: 10.1016/j.fm.2020.103462