dc.contributor.author	Kennedy, Aideen E.
dc.contributor.author	O'Mahony, Jim
dc.contributor.author	Byrne, Noel
dc.contributor.author	MacSharry, John
dc.contributor.author	Sayers, Riona G.
dc.date.accessioned	2017-12-08T13:33:47Z
dc.date.available	2017-12-08T13:33:47Z
dc.date.issued	2017-10-23
dc.description.abstract	The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 “Non-Friesian,” e.g., JerseyX, Norwegian Red) to assess IFN-γ levels and Mycobacterium avium subspecies paratuberculosis (MAP) antibody response before and after the bTB test. As part of the compulsory annual bTB test, avian and bovine PPD were administered at two separate cervical sites. To assess IFN-γ production, blood samples were taken before and 72 h after PPD administration. MAP antibody response was assessed before and 10 days post-PPD administration. A significant increase in MAP antibody response was identified post-bTB compared to pre-bTB response (p < 0.001). Additionally, IFN-γ production significantly increased at the post-bTB time point (p < 0.001) compared to the pre-bTB test readings. This may indicate a beneficial effect of bTB testing in controlling JD.	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	176
dc.identifier.citation	Kennedy, A. E., O’Mahony, J., Byrne, N., MacSharry, J. and Sayers, R. G. (2017) 'Is TB testing associated with increased blood interferon-gamma levels?', Frontiers in Veterinary Science, 4, 176 (9pp). doi: 10.3389/fvets.2017.00176	en
dc.identifier.doi	10.3389/fvets.2017.00176
dc.identifier.endpage	9
dc.identifier.issn	2297-1769
dc.identifier.journaltitle	Frontiers in Veterinary Science	en
dc.identifier.startpage	1
dc.identifier.uri	https://hdl.handle.net/10468/5153
dc.identifier.volume	4
dc.language.iso	en	en
dc.publisher	Frontiers Media	en
dc.relation.uri	https://www.frontiersin.org/articles/10.3389/fvets.2017.00176/full
dc.rights	© 2017, Kennedy, O’Mahony, Byrne, MacSharry and Sayers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Johne’s disease	en
dc.subject	TB test	en
dc.subject	Interferon-gamma	en
dc.subject	Purified protein derivative	en
dc.subject	ELISA	en
dc.title	Is TB testing associated with increased blood interferon-gamma levels?	en
dc.type	Article (peer-reviewed)	en

Is TB testing associated with increased blood interferon-gamma levels?

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