Determination of bifidobacterial carbohydrate utilization abilities and associated metabolic end products
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Date
03/03/2021
Authors
Solopova, Ana
van Sinderen, Douwe
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Publisher
Springer Science+Business Media, LLC
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Abstract
Bifidobacteria are able to utilize a diverse range of host-derived and dietary carbohydrates, the latter of which include many plant-derived oligo- and polysaccharides. Different bifidobacterial strains may possess different carbohydrate utilization abilities. These metabolic abilities can be studied using classical bacterial growth assessment methods, such as measurement of changes in optical density or acidity of the culture in the presence of the particular carbohydrate to generate growth and acidification curves, respectively. Scientists may also be interested in the growth rate during the exponential growth phase, and the maximum OD that is reached on a particular sugar, or the length of the lag phase. Furthermore, high-performance liquid chromatography (HPLC) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) are extensively used in carbohydrate and metabolic end-product analysis due to their versatility and separation capabilities.
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Keywords
Carbohydrate , Growth curve , Acidification , CFU , HPLC , HPAEC-PAD
Citation
Solopova, A. and van Sinderen, D. (2021) 'Determination of bifidobacterial carbohydrate utilization abilities and associated metabolic end products', in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 117-129. New York, NY: Humana Press. doi: 10.1007/978-1-0716-1274-3_10
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© 2021, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 117-129. New York, NY: Humana Press. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-1274-3_10