Determination of bifidobacterial carbohydrate utilization abilities and associated metabolic end products

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dc.contributor.authorSolopova, Ana
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.editorvan Sinderen, Douwe
dc.contributor.editorVentura, Marco
dc.date.accessioned2021-03-22T09:45:42Z
dc.date.available2021-03-22T09:45:42Z
dc.date.issued03/03/2021
dc.date.updated2021-03-19T12:59:23Z
dc.description.abstractBifidobacteria are able to utilize a diverse range of host-derived and dietary carbohydrates, the latter of which include many plant-derived oligo- and polysaccharides. Different bifidobacterial strains may possess different carbohydrate utilization abilities. These metabolic abilities can be studied using classical bacterial growth assessment methods, such as measurement of changes in optical density or acidity of the culture in the presence of the particular carbohydrate to generate growth and acidification curves, respectively. Scientists may also be interested in the growth rate during the exponential growth phase, and the maximum OD that is reached on a particular sugar, or the length of the lag phase. Furthermore, high-performance liquid chromatography (HPLC) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) are extensively used in carbohydrate and metabolic end-product analysis due to their versatility and separation capabilities.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSolopova, A. and van Sinderen, D. (2021) 'Determination of bifidobacterial carbohydrate utilization abilities and associated metabolic end products', in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 117-129. New York, NY: Humana Press. doi: 10.1007/978-1-0716-1274-3_10en
dc.identifier.doi10.1007/978-1-0716-1274-3_10en
dc.identifier.endpage129en
dc.identifier.isbn978-1-0716-1273-6
dc.identifier.isbn978-1-0716-1274-3
dc.identifier.issn1940-6029
dc.identifier.journaltitleMethods in Molecular Biologyen
dc.identifier.startpage117en
dc.identifier.urihttps://hdl.handle.net/10468/11153
dc.identifier.volume2278en
dc.language.isoenen
dc.publisherSpringer Science+Business Media, LLCen
dc.relation.ispartofBifidobacteria - Methods and Protocols
dc.relation.ispartofMethods in Molecular Biology, vol 2278
dc.rights© 2021, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 117-129. New York, NY: Humana Press. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-1274-3_10en
dc.subjectCarbohydrateen
dc.subjectGrowth curveen
dc.subjectAcidificationen
dc.subjectCFUen
dc.subjectHPLCen
dc.subjectHPAEC-PADen
dc.titleDetermination of bifidobacterial carbohydrate utilization abilities and associated metabolic end productsen
dc.typeBook chapteren
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