Mobilome and resistome reconstruction from genomes belonging to members of the Bifidobacterium genus

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dc.contributor.authorMancino, Walter
dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorVentura, Marco
dc.contributor.authorTurroni, Francesca
dc.contributor.funderHorizon 2020 Framework Programme
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2023-09-04T11:44:57Z
dc.date.available2023-09-04T11:19:43Zen
dc.date.available2023-09-04T11:44:57Z
dc.date.issued2019-12en
dc.date.updated2023-09-04T10:19:48Zen
dc.description.abstractSpecific members of the genus Bifidobacterium are among the first colonizers of the human/animal gut, where they act as important intestinal commensals associated with host health. As part of the gut microbiota, bifidobacteria may be exposed to antibiotics, used in particular for intrapartum prophylaxis, especially to prevent Streptococcus infections, or in the very early stages of life after the birth. In the current study, we reconstructed the in silico resistome of the Bifidobacterium genus, analyzing a database composed of 625 bifidobacterial genomes, including partial assembled strains with less than 100 genomic sequences. Furthermore, we screened bifidobacterial genomes for mobile genetic elements, such as transposases and prophage-like elements, in order to investigate the correlation between the bifido-mobilome and the bifido-resistome, also identifying genetic insertion hotspots that appear to be prone to horizontal gene transfer (HGT) events. These insertion hotspots were shown to be widely distributed among analyzed bifidobacterial genomes, and suggest the acquisition of antibiotic resistance genes through HGT events. These data were further corroborated by growth experiments directed to evaluate bacitracin A resistance in Bifidobacterium spp., a property that was predicted by in silico analyses to be part of the HGT-acquired resistome.en
dc.description.statusPeer revieweden
dc.description.versionPublished Version
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid638
dc.identifier.citationMancino, W., Lugli, G. A., Van Sinderen, D., Ventura, M., & Turroni, F. (2019). 'Mobilome and resistome reconstruction from genomes belonging to members of the bifidobacterium genus', Microorganisms, 7(12), 638, (15pp). doi: 10.3390/microorganisms7120638
dc.identifier.doi10.3390/microorganisms7120638en
dc.identifier.endpage15
dc.identifier.issued12
dc.identifier.journaltitleMicroorganisms
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/14902
dc.identifier.volume7
dc.language.isoenen
dc.publisherMDPI
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::CSA/696300/EU/The second coordination and support action for the JPI Healthy Diet for a Healthy Life/CSA JPI HDHL 2.0
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/EU Joint Programming Initiative::A Healthy Diet for a Healthy Life JPI (HDHL-JPI)/15/JP-HDHL/3280/IE/Impact of early life on microbiome development and later health (EarlyMicroHealth)/
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.rights© 2019 by the authors. LicenseeMDPI,Basel,Switzerland.Thisarticleisanopenaccess articledistributedunderthetermsandconditionsoftheCreativeCommonsAttribution (CCBY)license(http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBifidobacteria
dc.subjectGenomics
dc.subjectMobile elements
dc.subjectAntibiotic resistance genes
dc.titleMobilome and resistome reconstruction from genomes belonging to members of the Bifidobacterium genus
dc.typeArticle (peer-reviewed)
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