Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes

Bozorgzadeh, Somayyeh; Hamidi, Hassan; Ortiz, Roberto; Ludwig, Roland; Gorton, Lo

Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes

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Somayyeh_Bozorgzadeh_PCCP2015.pdf(1.55 MB)

Accepted version

c5cp03812j1.pdf(162.99 KB)

Supplementary information

Date

2015-08-24

Authors

Bozorgzadeh, Somayyeh

Hamidi, Hassan

Ortiz, Roberto

Ludwig, Roland

Gorton, Lo

Publisher

Royal Society of Chemistry

Published Version

10.1039/c5cp03812j

Abstract

In the present work, platinum and palladium nanoparticles (PtNPs and PdNPs) were decorated on the surface of multi-walled carbon nanotubes (MWCNTs) by a simple thermal decomposition method. The prepared nanohybrids, PtNPs–MWCNTs and PdNPs–MWCNTs, were cast on the surface of spectrographic graphite electrodes and then Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) was adsorbed on the modified layer. Direct electron transfer between PcCDH and the nanostructured modified electrodes was studied using flow injection amperometry and cyclic voltammetry. The maximum current responses (Imax) and the apparent Michaelis–Menten constants (KappM) for the different PcCDH modified electrodes were calculated by fitting the data to the Michaelis–Menten equation and compared. The sensitivity towards lactose was 3.07 and 3.28 μA mM−1 at the PcCDH/PtNPs–MWCNTs/SPGE and PcCDH/PdNPs–MWCNTs/SPGE electrodes, respectively, which were higher than those measured at the PcCDH/MWCNTs/SPGE (2.60 μA mM−1) and PcCDH/SPGE (0.92 μA mM−1). The modified electrodes were additionally tested as bioanodes for biofuel cell applications.

Keywords

Carbohydrate dehydrogenases , Electrodes , Electron transport , Metal nanoparticles , Nanotubes , Carbon , Palladium , Phanerochaete , Platinum , Surface properties , Temperature

Citation

Bozorgzadeh, S., Hamidi, H., Ortiz, R., Ludwig, R. and Gorton, L. (2015) 'Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes', Physical Chemistry Chemical Physics, 17 (37), pp. 24157-24165. doi: 10.1039/C5CP03812J