The Lactococcus lactis pan-plasmidome

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dc.contributor.authorKelleher, Philip
dc.contributor.authorMahony, Jennifer
dc.contributor.authorBottacini, Francesca
dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorVentura, Marco
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2019-11-19T10:14:21Z
dc.date.available2019-11-19T10:14:21Z
dc.date.issued2019-04-04
dc.description.abstractPlasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival. Genome sequencing of 16 Lactococcus lactis strains revealed the presence of 83 plasmids, including two megaplasmids. The limitations of Pacific Biosciences SMRT sequencing in detecting the total plasmid complement of lactococcal strains is examined, while a combined Illumina/SMRT sequencing approach is proposed to combat these issues. Comparative genome analysis of these plasmid sequences combined with other publicly available plasmid sequence data allowed the definition of the lactococcal plasmidome, and facilitated an investigation into (bio)technologically important plasmid-encoded traits such as conjugation, bacteriocin production, exopolysaccharide (EPS) production and (bacterio)phage resistance.en
dc.description.sponsorshipCheeseBoard 2015 project (10/RD/TMFRC/704)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid707en
dc.identifier.citationKelleher, P., Mahony, J., Bottacini, F., Lugli, G.A., Ventura, M. and Van Sinderen, D., 2019. The Lactococcus lactis pan-plasmidome. Frontiers in microbiology, 10. (19pp). DOI:10.3389/fmicb.2019.00707en
dc.identifier.doi10.3389/fmicb.2019.00707en
dc.identifier.eissn1664-302X
dc.identifier.endpage20en
dc.identifier.journaltitleFronti Miers incrobiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9048
dc.identifier.volume10en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2019.00707
dc.rights© 2019 Kelleher, Mahony, Bottacini, Lugli, Ventura and van Sinderenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectLactococcalen
dc.subjectPlasmiden
dc.subjectSMRT sequencingen
dc.subjectDairy fermentationen
dc.subjectConjugationen
dc.subjectLactic acid bacteriaen
dc.titleThe Lactococcus lactis pan-plasmidomeen
dc.typeArticle (peer-reviewed)en
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