The Lactococcus lactis pan-plasmidome
dc.contributor.author | Kelleher, Philip | |
dc.contributor.author | Mahony, Jennifer | |
dc.contributor.author | Bottacini, Francesca | |
dc.contributor.author | Lugli, Gabriele A. | |
dc.contributor.author | Ventura, Marco | |
dc.contributor.author | van Sinderen, Douwe | |
dc.contributor.funder | Science Foundation Ireland | en |
dc.contributor.funder | Department of Agriculture, Food and the Marine | en |
dc.date.accessioned | 2019-11-19T10:14:21Z | |
dc.date.available | 2019-11-19T10:14:21Z | |
dc.date.issued | 2019-04-04 | |
dc.description.abstract | Plasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival. Genome sequencing of 16 Lactococcus lactis strains revealed the presence of 83 plasmids, including two megaplasmids. The limitations of Pacific Biosciences SMRT sequencing in detecting the total plasmid complement of lactococcal strains is examined, while a combined Illumina/SMRT sequencing approach is proposed to combat these issues. Comparative genome analysis of these plasmid sequences combined with other publicly available plasmid sequence data allowed the definition of the lactococcal plasmidome, and facilitated an investigation into (bio)technologically important plasmid-encoded traits such as conjugation, bacteriocin production, exopolysaccharide (EPS) production and (bacterio)phage resistance. | en |
dc.description.sponsorship | CheeseBoard 2015 project (10/RD/TMFRC/704) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.articleid | 707 | en |
dc.identifier.citation | Kelleher, P., Mahony, J., Bottacini, F., Lugli, G.A., Ventura, M. and Van Sinderen, D., 2019. The Lactococcus lactis pan-plasmidome. Frontiers in microbiology, 10. (19pp). DOI:10.3389/fmicb.2019.00707 | en |
dc.identifier.doi | 10.3389/fmicb.2019.00707 | en |
dc.identifier.eissn | 1664-302X | |
dc.identifier.endpage | 20 | en |
dc.identifier.journaltitle | Fronti Miers incrobiology | en |
dc.identifier.startpage | 1 | en |
dc.identifier.uri | https://hdl.handle.net/10468/9048 | |
dc.identifier.volume | 10 | en |
dc.language.iso | en | en |
dc.publisher | Frontiers Media | en |
dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/ | en |
dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/ | en |
dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/ | en |
dc.relation.uri | https://www.frontiersin.org/articles/10.3389/fmicb.2019.00707 | |
dc.rights | © 2019 Kelleher, Mahony, Bottacini, Lugli, Ventura and van Sinderen | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Lactococcal | en |
dc.subject | Plasmid | en |
dc.subject | SMRT sequencing | en |
dc.subject | Dairy fermentation | en |
dc.subject | Conjugation | en |
dc.subject | Lactic acid bacteria | en |
dc.title | The Lactococcus lactis pan-plasmidome | en |
dc.type | Article (peer-reviewed) | en |