Riboflavin biosynthesis and overproduction by a derivative of the human gut commensal Bifidobacterium longum subsp. infantis ATCC 15697

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dc.contributor.authorSolopova, Anaen
dc.contributor.authorBottacini, Francescaen
dc.contributor.authorVenturi degli Esposti, Elenaen
dc.contributor.authorAmaretti, Albertoen
dc.contributor.authorRaimondi, Stefanoen
dc.contributor.authorRossi, Maddalenaen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.contributor.funderFederation of European Microbiological Societiesen
dc.date.accessioned2023-11-14T11:40:34Z
dc.date.available2023-11-14T11:40:34Z
dc.date.issued2020en
dc.description.abstractRiboflavin or vitamin B2 is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Despite increased interest in microbial synthesis of this water-soluble vitamin, the metabolic pathway for riboflavin biosynthesis has been characterized in just a handful of bacteria. Here, comparative genome analysis identified the genes involved in the de novo biosynthetic pathway of riboflavin in certain bifidobacterial species, including the human gut commensal Bifidobacterium longum subsp. infantis (B. infantis) ATCC 15697. Using comparative genomics and phylogenomic analysis, we investigated the evolutionary acquisition route of the riboflavin biosynthesis or rib gene cluster in Bifidobacterium and the distribution of riboflavin biosynthesis-associated genes across the genus. Using B. infantis ATCC 15697 as model organism for this pathway, we isolated spontaneous riboflavin overproducers, which had lost transcriptional regulation of the genes required for riboflavin biosynthesis. Among them, one mutant was shown to allow riboflavin release into the medium to a concentration of 60.8 ng mL−1. This mutant increased vitamin B2 concentration in a fecal fermentation system, thus providing promising data for application of this isolate as a functional food ingredient.en
dc.description.sponsorshipIrish Research Council (IRC Postdoctoral fellowship (GOIPD/2019/353))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid573335en
dc.identifier.citationSolopova, A., Bottacini, F., Venturi Degli Esposti, E., Amaretti, A., Raimondi, S., Rossi, M. and Van Sinderen, D. (2020) ‘Riboflavin biosynthesis and overproduction by a derivative of the human gut commensal bifidobacterium longum subsp. Infantis atcc 15697’, Frontiers in Microbiology, 11, 573335 (15pp). doi: 10.3389/fmicb.2020.573335en
dc.identifier.doi10.3389/fmicb.2020.573335en
dc.identifier.endpage15en
dc.identifier.issn1664-302Xen
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15227
dc.identifier.volume11en
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.ispartofFrontiers in Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectFEMS (Research Grant (FEMS-RG-2016-0103) and FEMS/ESCMID Award)en
dc.rights© 2020 Solopova, Bottacini, Venturi degli Esposti, Amaretti, Raimondi, Rossi and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. https://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectProbioticen
dc.subjectVitamin B2en
dc.subjectGut commensalen
dc.subjectVitamin biosynthesisen
dc.subjectHealth benefiten
dc.titleRiboflavin biosynthesis and overproduction by a derivative of the human gut commensal Bifidobacterium longum subsp. infantis ATCC 15697en
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.volume11en
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