Human in situ study of the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide immobilized in dental composite on controlling mature cariogenic biofilm

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dc.contributor.authorMelo, Mary Anne S.
dc.contributor.authorWeir, Michael D.
dc.contributor.authorPassos, Vanara F.
dc.contributor.authorRolim, Juliana P. M.
dc.contributor.authorLynch, Christopher D.
dc.contributor.authorRodrigues, Lidiany K. A.
dc.contributor.authorXu, Hockin H. K.
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológicoen
dc.contributor.funderCoordenação de Aperfeiçoamento de Pessoal de Nível Superioren
dc.contributor.funderUniversity of Maryland, Baltimore Countyen
dc.contributor.funderUniversity of Marylanden
dc.date.accessioned2019-08-07T09:29:29Z
dc.date.available2019-08-07T09:29:29Z
dc.date.issued2018-11-02
dc.description.abstractCariogenic oral biofilms cause recurrent dental caries around composite restorations, resulting in unprosperous oral health and expensive restorative treatment. Quaternary ammonium monomers that can be copolymerized with dental resin systems have been explored for the modulation of dental plaque biofilm growth over dental composite surfaces. Here, for the first time, we investigated the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide (QADM) on human overlying mature oral biofilms grown intra-orally in human participants for 7–14 days. Seventeen volunteers wore palatal devices containing composite specimens containing 10% by mass of QADM or a control composite without QADM. After 7 and 14 days, the adherent biofilms were collected to determine bacterial counts via colony-forming unit (CFU) counts. Biofilm viability, chronological changes, and percentage coverage were also determined through live/dead staining. QADM composites caused a significant inhibition of Streptococcus mutans biofilm formation for up to seven days. No difference in the CFU values were found for the 14-day period. Our findings suggest that: (1) QADM composites were successful in inhibiting 1–3-day biofilms in the oral environment in vivo; (2) QADM significantly reduced the portion of the S. mutans group; and (3) stronger antibiofilm activity is required for the control of mature long-term cariogenic biofilms. Contact-killing strategies using dental materials aimed at preventing or at least reducing high numbers of cariogenic bacteria seem to be a promising approach in patients at high risk of the recurrence of dental caries around composites.en
dc.description.sponsorshipCNPq (141791/2010-1); CAPES (BEX 0574/06-6); University of Maryland (Baltimore Seed grant, University of Maryland School of Dentistry bridge fund)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid3443en
dc.identifier.citationMelo, M., Weir, M., Passos, V., Rolim, J., Lynch, C., Rodrigues, L. and Xu, H., 2018. Human In Situ Study of the effect of Bis (2-Methacryloyloxyethyl) Dimethylammonium Bromide Immobilized in Dental Composite on Controlling Mature Cariogenic Biofilm. International journal of molecular sciences, 19(11):3443 (14pp.). DOI:10.3390/ijms19113443en
dc.identifier.doi10.3390/ijms19113443en
dc.identifier.eissn1422-0067
dc.identifier.endpage14en
dc.identifier.issn1661-6596
dc.identifier.issued11en
dc.identifier.journaltitleInternational Journal of Molecular Sciencesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8289
dc.identifier.volume19en
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.urihttps://www.mdpi.com/1422-0067/19/11/3443
dc.rights© 2018 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAntibacterialen
dc.subjectBiofilmen
dc.subjectCariesen
dc.subjectDental compositeen
dc.subjectQuaternary ammonium monomersen
dc.subjectHuman in situ studyen
dc.titleHuman in situ study of the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide immobilized in dental composite on controlling mature cariogenic biofilmen
dc.typeArticle (peer-reviewed)en
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