dc.contributor.author	Kennedy, Jonathan
dc.contributor.author	Flemer, Burkhardt
dc.contributor.author	Jackson, Stephen A.
dc.contributor.author	Morrissey, John P.
dc.contributor.author	O'Gara, Fergal
dc.contributor.author	Dobson, Alan D. W.
dc.contributor.funder	European Commission
dc.contributor.funder	Irish Government
dc.contributor.funder	Marine Institute
dc.date.accessioned	2016-02-17T11:44:40Z
dc.date.available	2016-02-17T11:44:40Z
dc.date.issued	2014
dc.description.abstract	The microbiota of four individual deep water sponges, Lissodendoryx diversichela, Poecillastra compressa, Inflatella pellicula, and Stelletta normani, together with surrounding seawater were analysed by pyrosequencing of a region of the 16S rRNA gene common to Bacteria and Archaea. Due to sampling constraints at depths below 700 m duplicate samples were not collected. The microbial communities of L. diversichela, P. compressa and I. pellicula were typical of low microbial abundance (LMA) sponges while S. normani had a community more typical of high microbial abundance (HMA) sponges. Analysis of the deep sea sponge microbiota revealed that the three LMA-like sponges shared a set of abundant OTUs that were distinct from those associated with sponges from shallow waters. Comparison of the pyrosequencing data with that from shallow water sponges revealed that the microbial communities of all sponges analysed have similar archaeal populations but that the bacterial populations of the deep sea sponges were distinct. Further analysis of the common and abundant OTUs from the three LMA-like sponges placed them within the groups of ammonia oxidising Archaea (Thaumarchaeota) and sulphur oxidising gamma-Proteobacteria (Chromatiales). Reads from these two groups made up over 70% of all 16S rRNA genes detected from the three LMA-like sponge samples, providing evidence of a putative common microbial assemblage associated with deep sea LMA sponges.	en
dc.description.sponsorship	PharmaSea project; EU Seventh Framework Programme (312184); Beuafort Marine Research Award; Marine Institute under the Marine Research Sub-Programme of the National Development Plan	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e91092
dc.identifier.citation	Kennedy J, Flemer B, Jackson SA, Morrissey JP, O'Gara F, Dobson ADW (2014) Evidence of a Putative Deep Sea Specific Microbiome in Marine Sponges. PLoS ONE 9(3): e91092. doi:10.1371/journal.pone.0091092
dc.identifier.doi	10.1371/journal.pone.0091092
dc.identifier.issn	1932-6203
dc.identifier.issued	3	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2340
dc.identifier.volume	9	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2015 Kennedy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	16S ribosomal RNA	en
dc.subject	Barrier Reef sponge	en
dc.subject	Antimicrobial activities	en
dc.subject	Irish waters	en
dc.subject	Cenarchaeum symbiosum	en
dc.subject	Phylogenetic analysis	en
dc.subject	Spatial distribution	en
dc.subject	Haliclona simulans	en
dc.subject	Abundance sponges	en
dc.subject	Sp. nov.	en
dc.title	Evidence of a putative deep sea specific microbiome in marine sponges	en
dc.type	Article (peer-reviewed)	en

Evidence of a putative deep sea specific microbiome in marine sponges

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